A method for the chemical phosphorylation of seryl residues in protamine has been developed. Both non-radioactive and [33P]phosphoprotamine have been prepared and characterized. Phosphoprotein phosphatases from a variety of mammalian tissues release inorganic phosphate from phosphoprotamine. Chemically phosphorylated protamine is a useful new substrate for the study of these phosphoprotein phosphatases.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 17 1975|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology