Chemical synaptic transmission onto superficial stellate cells of the mouse dorsal cochlear nucleus

Pierre F. Apostolides, Laurence Trussell

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The dorsal cochlear nucleus (DCN) is a cerebellum-like auditory brain stem region whose functions include sound localization and multisensory integration. Although previous in vivo studies have shown that glycinergic and GABAergic inhibition regulate the activity of several DCN cell types in response to sensory stimuli, data regarding the synaptic inputs onto DCN inhibitory interneurons remain limited. Using acute DCN slices from mice, we examined the properties of excitatory and inhibitory synapses onto the superficial stellate cell, a poorly understood cell type that provides inhibition to DCN output neurons (fusiform cells) as well as to local inhibitory interneurons (cartwheel cells). Excitatory synapses onto stellate cells activated both NMDA receptors and fast-gating, Ca2+-permeable AMPA receptors. Inhibition onto superficial stellate cells was mediated by glycine and GABAA receptors with different temporal kinetics. Paired recordings revealed that superficial stellate cells make reciprocal synapses and autapses, with a connection probability of _18-20%. Unexpectedly, superficial stellate cells co-released both glycine and GABA, suggesting that cotransmission may play a role in fine-tuning the duration of inhibitory transmission.

Original languageEnglish (US)
Pages (from-to)1812-1822
Number of pages11
JournalJournal of Neurophysiology
Volume111
Issue number9
DOIs
StatePublished - May 1 2014

Fingerprint

Cochlear Nucleus
Synaptic Transmission
Synapses
Interneurons
Sound Localization
Glycine Receptors
AMPA Receptors
GABA-A Receptors
N-Methyl-D-Aspartate Receptors
Glycine
Cerebellum
gamma-Aminobutyric Acid
Brain Stem
Neurons

Keywords

  • Auditory
  • Cochlear nucleus
  • Inhibition
  • Interneuron

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)
  • Medicine(all)

Cite this

Chemical synaptic transmission onto superficial stellate cells of the mouse dorsal cochlear nucleus. / Apostolides, Pierre F.; Trussell, Laurence.

In: Journal of Neurophysiology, Vol. 111, No. 9, 01.05.2014, p. 1812-1822.

Research output: Contribution to journalArticle

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