Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle

William T. Triplett, Celine Baligand, Sean C. Forbes, Rebecca J. Willcocks, Donovan J. Lott, Soren DeVos, Jim Pollaro, William Rooney, H. Lee Sweeney, Carsten G. Bönnemann, Dah Jyuu Wang, Krista Vandenborne, Glenn A. Walter

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Purpose The relationship between fat fractions (FFs) determined based on multiple TE, unipolar gradient echo images and 1H magnetic resonance spectroscopy (MRS) was evaluated using different models for fat-water decomposition, signal-to-noise ratios, and excitation flip angles. Methods A combination of single-voxel proton spectroscopy (1H-MRS) and gradient echo imaging was used to determine muscle FFs in both normal and dystrophic muscles. In order to cover a large range of FFs, the soleus and vastus lateralis muscles of 22 unaffected control subjects, 16 subjects with collagen VI deficiency (COL6), and 71 subjects with Duchenne muscular dystrophy (DMD) were studied. 1H-MRS-based FF were corrected for the increased muscle 1H2O T1 and T2 values observed in dystrophic muscles. Results Excellent agreement was found between coregistered FFs derived from gradient echo images fit to a multipeak model with noise bias correction and the relaxation-corrected 1H-MRS FFs (y = 0.93x + 0.003; R2 = 0.96) across the full range of FFs. Relaxation-corrected 1H-MRS FFs and imaging-based FFs were significantly elevated (P <0.01) in the muscles of COL6 and DMD subjects. Conclusion FFs, T2, and T1 were all sensitive to muscle involvement in dystrophic muscle. MRI offered an additional advantage over single-voxel spectroscopy in that the tissue heterogeneity in FFs could be readily determined.

Original languageEnglish (US)
Pages (from-to)8-19
Number of pages12
JournalMagnetic Resonance in Medicine
Volume72
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Skeletal Muscle
Fats
Muscles
Magnetic Resonance Spectroscopy
Duchenne Muscular Dystrophy
Spectrum Analysis
Quadriceps Muscle
Signal-To-Noise Ratio
Noise
Protons
Collagen
Water

Keywords

  • collagen VI
  • congenital muscular dystrophy
  • Duchenne muscular dystrophy
  • fat water imaging
  • magnetic resonance spectroscopy
  • muscle composition
  • skeletal muscle

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Triplett, W. T., Baligand, C., Forbes, S. C., Willcocks, R. J., Lott, D. J., DeVos, S., ... Walter, G. A. (2014). Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle. Magnetic Resonance in Medicine, 72(1), 8-19. https://doi.org/10.1002/mrm.24917

Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle. / Triplett, William T.; Baligand, Celine; Forbes, Sean C.; Willcocks, Rebecca J.; Lott, Donovan J.; DeVos, Soren; Pollaro, Jim; Rooney, William; Sweeney, H. Lee; Bönnemann, Carsten G.; Wang, Dah Jyuu; Vandenborne, Krista; Walter, Glenn A.

In: Magnetic Resonance in Medicine, Vol. 72, No. 1, 2014, p. 8-19.

Research output: Contribution to journalArticle

Triplett, WT, Baligand, C, Forbes, SC, Willcocks, RJ, Lott, DJ, DeVos, S, Pollaro, J, Rooney, W, Sweeney, HL, Bönnemann, CG, Wang, DJ, Vandenborne, K & Walter, GA 2014, 'Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle', Magnetic Resonance in Medicine, vol. 72, no. 1, pp. 8-19. https://doi.org/10.1002/mrm.24917
Triplett WT, Baligand C, Forbes SC, Willcocks RJ, Lott DJ, DeVos S et al. Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle. Magnetic Resonance in Medicine. 2014;72(1):8-19. https://doi.org/10.1002/mrm.24917
Triplett, William T. ; Baligand, Celine ; Forbes, Sean C. ; Willcocks, Rebecca J. ; Lott, Donovan J. ; DeVos, Soren ; Pollaro, Jim ; Rooney, William ; Sweeney, H. Lee ; Bönnemann, Carsten G. ; Wang, Dah Jyuu ; Vandenborne, Krista ; Walter, Glenn A. / Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle. In: Magnetic Resonance in Medicine. 2014 ; Vol. 72, No. 1. pp. 8-19.
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abstract = "Purpose The relationship between fat fractions (FFs) determined based on multiple TE, unipolar gradient echo images and 1H magnetic resonance spectroscopy (MRS) was evaluated using different models for fat-water decomposition, signal-to-noise ratios, and excitation flip angles. Methods A combination of single-voxel proton spectroscopy (1H-MRS) and gradient echo imaging was used to determine muscle FFs in both normal and dystrophic muscles. In order to cover a large range of FFs, the soleus and vastus lateralis muscles of 22 unaffected control subjects, 16 subjects with collagen VI deficiency (COL6), and 71 subjects with Duchenne muscular dystrophy (DMD) were studied. 1H-MRS-based FF were corrected for the increased muscle 1H2O T1 and T2 values observed in dystrophic muscles. Results Excellent agreement was found between coregistered FFs derived from gradient echo images fit to a multipeak model with noise bias correction and the relaxation-corrected 1H-MRS FFs (y = 0.93x + 0.003; R2 = 0.96) across the full range of FFs. Relaxation-corrected 1H-MRS FFs and imaging-based FFs were significantly elevated (P <0.01) in the muscles of COL6 and DMD subjects. Conclusion FFs, T2, and T1 were all sensitive to muscle involvement in dystrophic muscle. MRI offered an additional advantage over single-voxel spectroscopy in that the tissue heterogeneity in FFs could be readily determined.",
keywords = "collagen VI, congenital muscular dystrophy, Duchenne muscular dystrophy, fat water imaging, magnetic resonance spectroscopy, muscle composition, skeletal muscle",
author = "Triplett, {William T.} and Celine Baligand and Forbes, {Sean C.} and Willcocks, {Rebecca J.} and Lott, {Donovan J.} and Soren DeVos and Jim Pollaro and William Rooney and Sweeney, {H. Lee} and B{\"o}nnemann, {Carsten G.} and Wang, {Dah Jyuu} and Krista Vandenborne and Walter, {Glenn A.}",
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T1 - Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle

AU - Triplett, William T.

AU - Baligand, Celine

AU - Forbes, Sean C.

AU - Willcocks, Rebecca J.

AU - Lott, Donovan J.

AU - DeVos, Soren

AU - Pollaro, Jim

AU - Rooney, William

AU - Sweeney, H. Lee

AU - Bönnemann, Carsten G.

AU - Wang, Dah Jyuu

AU - Vandenborne, Krista

AU - Walter, Glenn A.

PY - 2014

Y1 - 2014

N2 - Purpose The relationship between fat fractions (FFs) determined based on multiple TE, unipolar gradient echo images and 1H magnetic resonance spectroscopy (MRS) was evaluated using different models for fat-water decomposition, signal-to-noise ratios, and excitation flip angles. Methods A combination of single-voxel proton spectroscopy (1H-MRS) and gradient echo imaging was used to determine muscle FFs in both normal and dystrophic muscles. In order to cover a large range of FFs, the soleus and vastus lateralis muscles of 22 unaffected control subjects, 16 subjects with collagen VI deficiency (COL6), and 71 subjects with Duchenne muscular dystrophy (DMD) were studied. 1H-MRS-based FF were corrected for the increased muscle 1H2O T1 and T2 values observed in dystrophic muscles. Results Excellent agreement was found between coregistered FFs derived from gradient echo images fit to a multipeak model with noise bias correction and the relaxation-corrected 1H-MRS FFs (y = 0.93x + 0.003; R2 = 0.96) across the full range of FFs. Relaxation-corrected 1H-MRS FFs and imaging-based FFs were significantly elevated (P <0.01) in the muscles of COL6 and DMD subjects. Conclusion FFs, T2, and T1 were all sensitive to muscle involvement in dystrophic muscle. MRI offered an additional advantage over single-voxel spectroscopy in that the tissue heterogeneity in FFs could be readily determined.

AB - Purpose The relationship between fat fractions (FFs) determined based on multiple TE, unipolar gradient echo images and 1H magnetic resonance spectroscopy (MRS) was evaluated using different models for fat-water decomposition, signal-to-noise ratios, and excitation flip angles. Methods A combination of single-voxel proton spectroscopy (1H-MRS) and gradient echo imaging was used to determine muscle FFs in both normal and dystrophic muscles. In order to cover a large range of FFs, the soleus and vastus lateralis muscles of 22 unaffected control subjects, 16 subjects with collagen VI deficiency (COL6), and 71 subjects with Duchenne muscular dystrophy (DMD) were studied. 1H-MRS-based FF were corrected for the increased muscle 1H2O T1 and T2 values observed in dystrophic muscles. Results Excellent agreement was found between coregistered FFs derived from gradient echo images fit to a multipeak model with noise bias correction and the relaxation-corrected 1H-MRS FFs (y = 0.93x + 0.003; R2 = 0.96) across the full range of FFs. Relaxation-corrected 1H-MRS FFs and imaging-based FFs were significantly elevated (P <0.01) in the muscles of COL6 and DMD subjects. Conclusion FFs, T2, and T1 were all sensitive to muscle involvement in dystrophic muscle. MRI offered an additional advantage over single-voxel spectroscopy in that the tissue heterogeneity in FFs could be readily determined.

KW - collagen VI

KW - congenital muscular dystrophy

KW - Duchenne muscular dystrophy

KW - fat water imaging

KW - magnetic resonance spectroscopy

KW - muscle composition

KW - skeletal muscle

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