Characterizing microscopic domains of birefringence in thin tissue sections

Steven Jacques, A. Moody, J. C. Ramella Roman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.D. Duncan, S.L. Jacques, P.C. Johnson
Pages464-468
Number of pages5
Volume4257
DOIs
StatePublished - 2001
EventLaser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical - San Jose, CA, United States
Duration: Jan 21 2001Jan 24 2001

Other

OtherLaser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical
CountryUnited States
CitySan Jose, CA
Period1/21/011/24/01

Fingerprint

Birefringence
birefringence
Light measurement
Tissue
Light polarization
Tissue engineering
Liver
Laser beams
Muscle
Skin
Microscopes
tissue engineering
Monomers
Pixels
Cameras
Polymerization
muscles
Proteins
optical measurement
liver

Keywords

  • Fiber orientation
  • Liver
  • Muscle
  • Polarization
  • Skin

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Jacques, S., Moody, A., & Ramella Roman, J. C. (2001). Characterizing microscopic domains of birefringence in thin tissue sections. In D. D. Duncan, S. L. Jacques, & P. C. Johnson (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4257, pp. 464-468) https://doi.org/10.1117/12.434735

Characterizing microscopic domains of birefringence in thin tissue sections. / Jacques, Steven; Moody, A.; Ramella Roman, J. C.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.D. Duncan; S.L. Jacques; P.C. Johnson. Vol. 4257 2001. p. 464-468.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jacques, S, Moody, A & Ramella Roman, JC 2001, Characterizing microscopic domains of birefringence in thin tissue sections. in DD Duncan, SL Jacques & PC Johnson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4257, pp. 464-468, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, San Jose, CA, United States, 1/21/01. https://doi.org/10.1117/12.434735
Jacques S, Moody A, Ramella Roman JC. Characterizing microscopic domains of birefringence in thin tissue sections. In Duncan DD, Jacques SL, Johnson PC, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4257. 2001. p. 464-468 https://doi.org/10.1117/12.434735
Jacques, Steven ; Moody, A. ; Ramella Roman, J. C. / Characterizing microscopic domains of birefringence in thin tissue sections. Proceedings of SPIE - The International Society for Optical Engineering. editor / D.D. Duncan ; S.L. Jacques ; P.C. Johnson. Vol. 4257 2001. pp. 464-468
@inproceedings{cc4c569a5aaf420f958f6ae1ed0359d0,
title = "Characterizing microscopic domains of birefringence in thin tissue sections",
abstract = "A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.",
keywords = "Fiber orientation, Liver, Muscle, Polarization, Skin",
author = "Steven Jacques and A. Moody and {Ramella Roman}, {J. C.}",
year = "2001",
doi = "10.1117/12.434735",
language = "English (US)",
volume = "4257",
pages = "464--468",
editor = "D.D. Duncan and S.L. Jacques and P.C. Johnson",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Characterizing microscopic domains of birefringence in thin tissue sections

AU - Jacques, Steven

AU - Moody, A.

AU - Ramella Roman, J. C.

PY - 2001

Y1 - 2001

N2 - A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.

AB - A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.

KW - Fiber orientation

KW - Liver

KW - Muscle

KW - Polarization

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0034864740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034864740&partnerID=8YFLogxK

U2 - 10.1117/12.434735

DO - 10.1117/12.434735

M3 - Conference contribution

AN - SCOPUS:0034864740

VL - 4257

SP - 464

EP - 468

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Duncan, D.D.

A2 - Jacques, S.L.

A2 - Johnson, P.C.

ER -