Characterizing extravascular neutrophil migration in vivo in the iris

Stephen Planck, Matthias D. Becker, Sergio Crespo, Dongseok Choi, Kellen Galster, Kiera L. Garman, Rainer Nobiling, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Extravascular neutrophil migration is poorly characterized in vivo. To test the hypothesis that this migration is a non-random process, we used videomicroscopy to monitor neutrophils in irises of living mice with endotoxin-induced uveitis (EIU). Paths of individual cells were analyzed. Nearly all of these cells were moving in divergent directions, and mean displacement plots indicated that the predominant movement was random. The paths of some cells were fit to bivariate autoregressive integrated moving average models that revealed at least two modes of movement: random search and linear trend. Cell speed was significantly reduced by the actin inhibitor, cytochalasin D. The pattern of migration for neutrophils is in marked contrast to what we previously described for antigen-presenting cells in the iris, but somewhat resembles recent descriptions for T cells within a lymph node. Characterization of extravascular migration of neutrophils has important implications for understanding infection and immunity.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalInflammation
Volume31
Issue number2
DOIs
StatePublished - Apr 2008

Fingerprint

Iris
Neutrophils
Cytochalasin D
Video Microscopy
Uveitis
Antigen-Presenting Cells
Endotoxins
Actins
Immunity
Lymph Nodes
T-Lymphocytes
Infection

Keywords

  • Intravital microscopy
  • Leukocyte trafficking
  • Uveitis

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Characterizing extravascular neutrophil migration in vivo in the iris. / Planck, Stephen; Becker, Matthias D.; Crespo, Sergio; Choi, Dongseok; Galster, Kellen; Garman, Kiera L.; Nobiling, Rainer; Rosenbaum, James (Jim).

In: Inflammation, Vol. 31, No. 2, 04.2008, p. 105-111.

Research output: Contribution to journalArticle

Planck, Stephen ; Becker, Matthias D. ; Crespo, Sergio ; Choi, Dongseok ; Galster, Kellen ; Garman, Kiera L. ; Nobiling, Rainer ; Rosenbaum, James (Jim). / Characterizing extravascular neutrophil migration in vivo in the iris. In: Inflammation. 2008 ; Vol. 31, No. 2. pp. 105-111.
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