Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7

Ann Hill, A. Worth, T. Elliott, S. Rowland-Jones, J. Brooks, A. Rickinson, A. McMichael

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides. Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. This behavior contrasted with HLA-B51, which binds peptides with a similar motif to B7, and has identical amino acid residues at sites expected to form the 'F' pocket of the peptide-binding groove. HLA-B51 also bound the peptide EBNA 3C 881-9, but was unable to bind peptides extended at the C terminus.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalEuropean Journal of Immunology
Volume25
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

HLA-B7 Antigen
Human Herpesvirus 4
Epitopes
Peptides
HLA-B51 Antigen
Cytotoxic T-Lymphocytes
Detergents
Alleles
Amino Acids

Keywords

  • Epstein-Barr virus
  • HLA-B7
  • Peptide binding

ASJC Scopus subject areas

  • Immunology

Cite this

Hill, A., Worth, A., Elliott, T., Rowland-Jones, S., Brooks, J., Rickinson, A., & McMichael, A. (1995). Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. European Journal of Immunology, 25(1), 18-24.

Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. / Hill, Ann; Worth, A.; Elliott, T.; Rowland-Jones, S.; Brooks, J.; Rickinson, A.; McMichael, A.

In: European Journal of Immunology, Vol. 25, No. 1, 1995, p. 18-24.

Research output: Contribution to journalArticle

Hill, A, Worth, A, Elliott, T, Rowland-Jones, S, Brooks, J, Rickinson, A & McMichael, A 1995, 'Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7', European Journal of Immunology, vol. 25, no. 1, pp. 18-24.
Hill A, Worth A, Elliott T, Rowland-Jones S, Brooks J, Rickinson A et al. Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. European Journal of Immunology. 1995;25(1):18-24.
Hill, Ann ; Worth, A. ; Elliott, T. ; Rowland-Jones, S. ; Brooks, J. ; Rickinson, A. ; McMichael, A. / Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. In: European Journal of Immunology. 1995 ; Vol. 25, No. 1. pp. 18-24.
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