Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase

Masayuki Otsu, Ian Hiles, Ivan Gout, Michael J. Fry, Fernanda Ruiz-Larrea, George Panayotou, Andrew Thompson, Ritu Dhand, Justin Hsuan, Nicholas Totty, Anthony D. Smith, Sarah J. Morgan, Sara Courtneidge, Peter J. Parker, Michael D. Waterfield

Research output: Contribution to journalArticle

522 Citations (Scopus)

Abstract

Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85α and p85β), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85β immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85α or p85β and the 110 kd protein binds to PDGF but not EGF receptors. p85α and p85β may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.

Original languageEnglish (US)
Pages (from-to)91-104
Number of pages14
JournalCell
Volume65
Issue number1
DOIs
StatePublished - Apr 5 1991
Externally publishedYes

Fingerprint

Phosphatidylinositol 3-Kinase
Receptor Protein-Tyrosine Kinases
Proteins
Polyomavirus
Cloning
Viral Tumor Antigens
Protein Sequence Analysis
Viruses
Protein-Tyrosine Kinases
Tyrosine
Organism Cloning
Immune Sera
Brain
Phosphotransferases
Complementary DNA
Amino Acids
Substrates

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Otsu, M., Hiles, I., Gout, I., Fry, M. J., Ruiz-Larrea, F., Panayotou, G., ... Waterfield, M. D. (1991). Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. Cell, 65(1), 91-104. https://doi.org/10.1016/0092-8674(91)90411-Q

Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. / Otsu, Masayuki; Hiles, Ian; Gout, Ivan; Fry, Michael J.; Ruiz-Larrea, Fernanda; Panayotou, George; Thompson, Andrew; Dhand, Ritu; Hsuan, Justin; Totty, Nicholas; Smith, Anthony D.; Morgan, Sarah J.; Courtneidge, Sara; Parker, Peter J.; Waterfield, Michael D.

In: Cell, Vol. 65, No. 1, 05.04.1991, p. 91-104.

Research output: Contribution to journalArticle

Otsu, M, Hiles, I, Gout, I, Fry, MJ, Ruiz-Larrea, F, Panayotou, G, Thompson, A, Dhand, R, Hsuan, J, Totty, N, Smith, AD, Morgan, SJ, Courtneidge, S, Parker, PJ & Waterfield, MD 1991, 'Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase', Cell, vol. 65, no. 1, pp. 91-104. https://doi.org/10.1016/0092-8674(91)90411-Q
Otsu, Masayuki ; Hiles, Ian ; Gout, Ivan ; Fry, Michael J. ; Ruiz-Larrea, Fernanda ; Panayotou, George ; Thompson, Andrew ; Dhand, Ritu ; Hsuan, Justin ; Totty, Nicholas ; Smith, Anthony D. ; Morgan, Sarah J. ; Courtneidge, Sara ; Parker, Peter J. ; Waterfield, Michael D. / Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. In: Cell. 1991 ; Vol. 65, No. 1. pp. 91-104.
@article{07078256823d4275bdf971f210814fcd,
title = "Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase",
abstract = "Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85α and p85β), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85β immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85α or p85β and the 110 kd protein binds to PDGF but not EGF receptors. p85α and p85β may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.",
author = "Masayuki Otsu and Ian Hiles and Ivan Gout and Fry, {Michael J.} and Fernanda Ruiz-Larrea and George Panayotou and Andrew Thompson and Ritu Dhand and Justin Hsuan and Nicholas Totty and Smith, {Anthony D.} and Morgan, {Sarah J.} and Sara Courtneidge and Parker, {Peter J.} and Waterfield, {Michael D.}",
year = "1991",
month = "4",
day = "5",
doi = "10.1016/0092-8674(91)90411-Q",
language = "English (US)",
volume = "65",
pages = "91--104",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase

AU - Otsu, Masayuki

AU - Hiles, Ian

AU - Gout, Ivan

AU - Fry, Michael J.

AU - Ruiz-Larrea, Fernanda

AU - Panayotou, George

AU - Thompson, Andrew

AU - Dhand, Ritu

AU - Hsuan, Justin

AU - Totty, Nicholas

AU - Smith, Anthony D.

AU - Morgan, Sarah J.

AU - Courtneidge, Sara

AU - Parker, Peter J.

AU - Waterfield, Michael D.

PY - 1991/4/5

Y1 - 1991/4/5

N2 - Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85α and p85β), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85β immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85α or p85β and the 110 kd protein binds to PDGF but not EGF receptors. p85α and p85β may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.

AB - Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85α and p85β), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85β immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85α or p85β and the 110 kd protein binds to PDGF but not EGF receptors. p85α and p85β may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.

UR - http://www.scopus.com/inward/record.url?scp=0025755422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025755422&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(91)90411-Q

DO - 10.1016/0092-8674(91)90411-Q

M3 - Article

C2 - 1707345

AN - SCOPUS:0025755422

VL - 65

SP - 91

EP - 104

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -