Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase

Masayuki Otsu, Ian Hiles, Ivan Gout, Michael J. Fry, Fernanda Ruiz-Larrea, George Panayotou, Andrew Thompson, Ritu Dhand, Justin Hsuan, Nicholas Totty, Anthony D. Smith, Sarah J. Morgan, Sara A. Courtneidge, Peter J. Parker, Michael D. Waterfield

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530 Scopus citations

Abstract

Affinity-purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) contains two major proteins of 85 and 110 kd. Amino acid sequence analysis and cDNA cloning reveals two related 85 kd proteins (p85α and p85β), which both contain one SH3 and two SH2 regions (src homology regions). When expressed, these 85 kd proteins bind to and are substrates for tyrosine-phosphorylated receptor kinases and the polyoma virus middle-T antigen/pp60c-src complex, but lack PI3-kinase activity. However, an antiserum raised against p85β immunoprecipitates PI3-kinase activity. The active PI3-kinase complex containing p85α or p85β and the 110 kd protein binds to PDGF but not EGF receptors. p85α and p85β may mediate specific PI3-kinase interactions with a subset of tyrosine kinases.

Original languageEnglish (US)
Pages (from-to)91-104
Number of pages14
JournalCell
Volume65
Issue number1
DOIs
StatePublished - Apr 5 1991

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Otsu, M., Hiles, I., Gout, I., Fry, M. J., Ruiz-Larrea, F., Panayotou, G., Thompson, A., Dhand, R., Hsuan, J., Totty, N., Smith, A. D., Morgan, S. J., Courtneidge, S. A., Parker, P. J., & Waterfield, M. D. (1991). Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. Cell, 65(1), 91-104. https://doi.org/10.1016/0092-8674(91)90411-Q