Abstract
Analogs of the TRα-specific thyromimetic CO23 were synthesized and analyzed in vitro using competitive binding and transactivation assays. Like CO23, all analogs bind to both thyroid hormone receptor subtypes with about the same affinity; however, modification of CO23 by derivatization of the 3′ position of the outer-ring or replacement of the inner-ring iodides with bromides attenuates binding. Despite lacking a preference in binding to TRα, all analogs display TRα-specificity in transactivation assays using U2OS and HeLa cells. At best, several agonists exhibit an approximately 6-12-fold preference in transactivation when tested with TRα in HeLa cells. One analog, CO24, showed in vivo TRα-specific action in a tadpole metamorphosis assay.
Original language | English (US) |
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Pages (from-to) | 762-770 |
Number of pages | 9 |
Journal | Bioorganic and Medicinal Chemistry |
Volume | 16 |
Issue number | 2 |
DOIs | |
State | Published - Jan 15 2008 |
Keywords
- CO23
- CO23 analogs
- Cardiac specific
- GC-1
- In vivo
- Induced metamorphosis
- Liver specific
- Metamorphosis
- Thyroid hormone receptor α
- Thyroid hormone receptor β
- Thyroid hormone receptors
- Xenopus laevis
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry