Characterization of the role rab25 in energy metabolism and cancer using extracellular flux analysis and material balance

Shreya Mitra, Jennifer Molina, Gordon B. Mills, Jennifer B.D. Ennison

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Rab25, by altering traffi cking of critical cellular resources, infl uences cell metabolism and survival during stress conditions. Overall, perturbations in the vesicular traffi cking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidifi cation Rate, ECAR (glycolysis) by an extracellular fl ux analyzer (XF96, Seahorse Biosciences, MA). Additionally, overall turnover of glucose, lactate, as well as glutamine and glutamate can be measured biochemically using the YSI2900 Biochemistry Analyzer (YSI Incorporated, Life Sciences, OH). A combination of these two methods allows a precise and quantitative approach to interrogate the role of Rab25 as well as other Rab GTPases in central carbon energy metabolism.

Original languageEnglish (US)
Pages (from-to)195-205
Number of pages11
JournalMethods in Molecular Biology
Volume1298
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Breast cancer
  • Extracellular flux
  • Glucose
  • Glutamine
  • Glycolytic index
  • Lactate
  • Rab25
  • XF 96 extracellular flux analyzer
  • YSI biochemistry analyzer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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