TY - JOUR
T1 - Characterization of the role rab25 in energy metabolism and cancer using extracellular flux analysis and material balance
AU - Mitra, Shreya
AU - Molina, Jennifer
AU - Mills, Gordon B.
AU - Ennison, Jennifer B.D.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Rab25, by altering traffi cking of critical cellular resources, infl uences cell metabolism and survival during stress conditions. Overall, perturbations in the vesicular traffi cking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidifi cation Rate, ECAR (glycolysis) by an extracellular fl ux analyzer (XF96, Seahorse Biosciences, MA). Additionally, overall turnover of glucose, lactate, as well as glutamine and glutamate can be measured biochemically using the YSI2900 Biochemistry Analyzer (YSI Incorporated, Life Sciences, OH). A combination of these two methods allows a precise and quantitative approach to interrogate the role of Rab25 as well as other Rab GTPases in central carbon energy metabolism.
AB - Rab25, by altering traffi cking of critical cellular resources, infl uences cell metabolism and survival during stress conditions. Overall, perturbations in the vesicular traffi cking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidifi cation Rate, ECAR (glycolysis) by an extracellular fl ux analyzer (XF96, Seahorse Biosciences, MA). Additionally, overall turnover of glucose, lactate, as well as glutamine and glutamate can be measured biochemically using the YSI2900 Biochemistry Analyzer (YSI Incorporated, Life Sciences, OH). A combination of these two methods allows a precise and quantitative approach to interrogate the role of Rab25 as well as other Rab GTPases in central carbon energy metabolism.
KW - Breast cancer
KW - Extracellular flux
KW - Glucose
KW - Glutamine
KW - Glycolytic index
KW - Lactate
KW - Rab25
KW - XF 96 extracellular flux analyzer
KW - YSI biochemistry analyzer
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UR - http://www.scopus.com/inward/citedby.url?scp=84925967503&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2569-8_17
DO - 10.1007/978-1-4939-2569-8_17
M3 - Article
C2 - 25800844
AN - SCOPUS:84925967503
SN - 1064-3745
VL - 1298
SP - 195
EP - 205
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -