Characterization of the role for calcium influx in mitogen‐induced triggering of human T cells. Identification of calcium‐dependent and calcium‐independent signals

Erwin W. Gelfand, Roy K. Cheung, Sergio Grinstein, Gordon B. Mills

    Research output: Contribution to journalArticle

    74 Scopus citations

    Abstract

    Entry of Ca2+ into the cell is recognized as an important activation signal for mitogeninduced lymphocyte proliferation. Changes in free cytosolic Ca2+ ([Ca2+]i) can now be measured directly using the fluorescent reagent quin‐2. To analyze the role of [Ca2+]i in human T cell activation, we have determined the effect of the calcium channel blocker, nifedipine, on phytohemagglutinin (PHA)‐induced lymphocyte proliferation. At a concentration of 50 μM, nifedipine is nontoxic, and prevents PHA‐induced proliferation. In parallel the drug prevents the lectin‐induced increase in concentration of [Ca2+]i and interleukin 2 (IL2) secretion; IL2 receptor expression is unaffected. In the presence of exogenous IL2, cell proliferation proceeds normally. Treatment of the cells with the tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate prevents the inhibitory effect of nifedipine on cell proliferation. Since TPA is itself nonmitogenic and does not affect levels of cytosolic Ca2+, these data and the data on IL2 receptor expression indicate that PHA can generate an activation signal(s) which is [Ca2+]i independent.

    Original languageEnglish (US)
    Pages (from-to)907-912
    Number of pages6
    JournalEuropean Journal of Immunology
    Volume16
    Issue number8
    DOIs
    StatePublished - 1986

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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