Characterization of specific CD4 and CD8 T-cell responses in QuantiFERON TB Gold-Plus TB1 and TB2 tubes

Nadia PC Allen, Gwendolyn Swarbrick, Meghan Cansler, Megan Null, Hiam Salim, Misato Miyamasu, Jenny Howard, Jeffrey Boyle, David Lewinsohn, Deborah Lewinsohn

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The new generation of the IGRA QuantiFERON-TB Gold Plus (QFT-Plus) includes two antigen tubes, TB1 and TB2 which contain specific Mycobacterium tuberculosis peptides designed to stimulate both CD4 and CD8 T-cells. TB1 is designed to target cell mediated responses from CD4 T-cells, while TB2 contains newly designed peptides designed to also stimulate CD8 T-cells. We identified specific CD4 and CD8 T-cell clones that recognize different regions spanning the length of the CFP-10 protein in M. tuberculosisto directly test in QFT-Plus TB1 and TB2 tubes, followed by Interferon-gamma detection by the QFT-Plus ELISA. These clones showed specific responses to the different QFT-Plus tubes, the CD4 T-cell clone showed dose-dependent responses to both TB1 and TB2 tubes, while the CD8 T-cell clones showed specific and targeted responses to the QFT-Plus TB2 tube (>140-fold difference) versus the QFT-Plus TB1 tubes using the QFT-Plus ELISA. This testing provides direct evidence of the specificity of CD8 T-cell mediated response in QFT-Plus TB2 tubes.

Original languageEnglish (US)
Pages (from-to)239-241
Number of pages3
JournalTuberculosis
Volume113
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Keywords

  • CD4 T-cell responses
  • CD8 T-cell responses
  • QFT-Plus
  • QuantiFERON
  • Tuberculosis (TB)

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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    Allen, N. PC., Swarbrick, G., Cansler, M., Null, M., Salim, H., Miyamasu, M., Howard, J., Boyle, J., Lewinsohn, D., & Lewinsohn, D. (2018). Characterization of specific CD4 and CD8 T-cell responses in QuantiFERON TB Gold-Plus TB1 and TB2 tubes. Tuberculosis, 113, 239-241. https://doi.org/10.1016/j.tube.2018.10.014