Characterization of ResDE-dependent fnr transcription in Bacillus subtilis

Hao Geng, Yi Zhu, Karl Mullen, Cole S. Zuber, Michiko Nakano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The ResD-ResE signal transduction system is required for transcription of genes involved in aerobic and anaerobic respiration in Bacillus subtilis. Phosphorylated ResD (ResD∼P) interacts with target DNA to activate transcription. A strong sequence similarity was detected in promoter regions of some ResD-controlled genes including fnr and resA. Single-base substitutions in the fnr and resA promoters were performed to determine a ResD-binding sequence. DNase I footprinting analysis indicated that ResD∼P itself does not bind to fnr, but interaction of ResD∼P with the C-terminal domain of the α subunit (αCTD) of RNA polymerase (RNAP) facilitates cooperative binding of ResD∼P and RNAP, thereby increasing fnr transcription initiation. Consistent with this result, amino acid substitutions in αCTD, such as Y263A, K267A, A269I, or N290A, sharply reduced fnr transcription in vivo, and the K267A αCTD protein, unlike the wild-type protein, did not increase ResD∼P binding to the fnr promoter. Amino acid residues of αCTD required for ResD-dependent fnr transcription, with the exception of N290, which may interact with DNA, constitute a distinct surface, suggesting that these residues likely interact with ResD∼P.

Original languageEnglish (US)
Pages (from-to)1745-1755
Number of pages11
JournalJournal of Bacteriology
Volume189
Issue number5
DOIs
StatePublished - Mar 2007

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DNA-Directed RNA Polymerases
Bacillus subtilis
Deoxyribonuclease I
DNA
Protein Subunits
Amino Acid Substitution
Genetic Promoter Regions
Genes
Signal Transduction
Respiration
Amino Acids
Proteins
Protein Domains

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Characterization of ResDE-dependent fnr transcription in Bacillus subtilis. / Geng, Hao; Zhu, Yi; Mullen, Karl; Zuber, Cole S.; Nakano, Michiko.

In: Journal of Bacteriology, Vol. 189, No. 5, 03.2007, p. 1745-1755.

Research output: Contribution to journalArticle

Geng, Hao ; Zhu, Yi ; Mullen, Karl ; Zuber, Cole S. ; Nakano, Michiko. / Characterization of ResDE-dependent fnr transcription in Bacillus subtilis. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 5. pp. 1745-1755.
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