Characterization of Pyruvate Uptake in Escherichia coli K-12

Jens Kreth, Joseph W. Lengeler, Knut Jahreis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The monocarboxylate pyruvate is an important metabolite and can serve as sole carbon source for Escherichia coli. Although specific pyruvate transporters have been identified in two bacterial species, pyruvate transport is not well understood in E. coli. In the present study, pyruvate transport was investigated under different growth conditions. The transport of pyruvate shows specific activities depending on the growth substrate used as sole carbon source, suggesting the existence of at least two systems for pyruvate uptake: i) one inducible system and probably highly specific for pyruvate and ii) one system active under non-induced conditions. Using the toxic pyruvate analog 3-fluoropyruvate, a mutant was isolated unable to grow on and transport pyruvate. Further investigation revealed that a revertant selected for growth on pyruvate regained the inducible pyruvate transport activity. Characterization of pyruvate excretion showed that the pyruvate transport negative mutant accumulated pyruvate in the growth medium suggesting an additional transport system for pyruvate excretion. The here presented data give valuable insight into the pyruvate metabolism and transport of E. coli suggesting the presence of at least two uptake systems and one excretion system to balance the intracellular level of pyruvate.

Original languageEnglish (US)
Article numbere67125
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 20 2013
Externally publishedYes

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Escherichia coli K12
Pyruvic Acid
Escherichia coli
excretion
uptake mechanisms
mutants
carbon
transporters
culture media
metabolites
metabolism
Growth
Carbon
Poisons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Characterization of Pyruvate Uptake in Escherichia coli K-12. / Kreth, Jens; Lengeler, Joseph W.; Jahreis, Knut.

In: PLoS One, Vol. 8, No. 6, e67125, 20.06.2013.

Research output: Contribution to journalArticle

Kreth, Jens ; Lengeler, Joseph W. ; Jahreis, Knut. / Characterization of Pyruvate Uptake in Escherichia coli K-12. In: PLoS One. 2013 ; Vol. 8, No. 6.
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