Characterization of prenylated protein methyltransferase in Leishmania

Marie Pierre Hasne, Françoise Lawrence

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prenylated protein methyltransferase, an enzyme involved in the post-translational modification of many signalling proteins, has been characterized in a parasitic flagellated protozoan, Leishmania donovani. The activity of this enzyme was monitored by the methylation of an artificial substrate, an S-prenylated cysteine analogue, with S-adenosyl-L-[methyl-3H]methionine as methyl donor. More than 85% of the methyltransferase activity was associated with membranes. The enzyme methylates N-acetyl-S-trans, trans-farnesyl-L-cysteine and N-acetyl-S-all-trans-geranylgeranyl-L-cysteine, but N-acetyl-S-trans, trans-geranyl-L-cysteine only very weakly. In contrast with the enzyme from mammals, the leishmanial enzyme had a greater affinity for the farnesylated substrate than for the geranylgeranylated one. Activity in vitro was not modulated by cAMP, protein kinase C activator or guanosine 5'-[γ-thio]triphosphate. An analysis of the endo gene us substrates showed that the carboxymethylated proteins were also isoprenylated. The main carboxymethylated proteins have molecular masses of 95, 68, 55, 46, 34-23, 18 and less than 14 kDa. Treatment of cells with N-acetyl-S-trans, trans-farnesyl-L-cysteine decreased the carboxymethylation level, whereas treatment with guanosine 5'-[γ-thio]triphosphate increased the carboxymethylation of various proteins, particularly those of molecular masses 30-20 kDa.

Original languageEnglish (US)
Pages (from-to)513-518
Number of pages6
JournalBiochemical Journal
Volume342
Issue number3
DOIs
StatePublished - Sep 15 1999
Externally publishedYes

Fingerprint

Protein Methyltransferases
Leishmania
Enzymes
Guanosine
Molecular mass
Cysteine
Proteins
Substrates
Leishmania donovani
Methylation
Mammals
Methyltransferases
Post Translational Protein Processing
Protein Kinase C
Genes
Cells
Membranes

Keywords

  • Kinetoplastidae
  • Leishmania donovani
  • Promastigote
  • Protozoans
  • Sinefungin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of prenylated protein methyltransferase in Leishmania. / Pierre Hasne, Marie; Lawrence, Françoise.

In: Biochemical Journal, Vol. 342, No. 3, 15.09.1999, p. 513-518.

Research output: Contribution to journalArticle

Pierre Hasne, Marie ; Lawrence, Françoise. / Characterization of prenylated protein methyltransferase in Leishmania. In: Biochemical Journal. 1999 ; Vol. 342, No. 3. pp. 513-518.
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