Characterization of pp85, a Target of Oncogenes and Growth Factor Receptors

Bruce Cohen, Yuxi Liu, Brian Druker, Thomas M. Roberts, Brian S. Schaffhausen

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

An 85,000-molecular-weight polypeptide (85K polypeptide) has previously been identified as a common substrate for tyrosine phosphorylation upon polyomavirus middle T transformation or upon platelet-derived growth factor stimulation of 3T3 cells. In each case, pp85 has an associated phosphatidylinositol kinase activity. The tissue distribution of pp85 was determined by middle T blotting experiments; the highest levels were found in brain, lung, and spleen tissues. High-resolution examination of 85K by isoelectric focusing demonstrated that there are at least 10 different forms. These were resolved into two families, 85K and 86K; the ratio of the two families changed in different cells. Similar forms were found for pp85 associated with pp60v-src. Individual species within each family differed by phosphorylation. Analysis of pp85 and pp86 by immunoprecipitation with anti-phosphotyrosine antibody showed increasing phosphorylation in response to middle T or pp60v-src transformation. The association of middle T with pp85 and pp60c-src was examined in pulse-chase experiments. Association of middle T with pp60c-src was slow and was accompanied by progressive modification of middle T. pp85 formed a dissociable complex with middle T within 2.5 min.

Original languageEnglish (US)
Pages (from-to)2909-2915
Number of pages7
JournalMolecular and Cellular Biology
Volume10
Issue number6
StatePublished - Jun 1990
Externally publishedYes

Fingerprint

Growth Factor Receptors
Oncogenes
Phosphorylation
3T3 Cells
Polyomavirus
Peptides
Phosphotyrosine
Platelet-Derived Growth Factor
Isoelectric Focusing
Tissue Distribution
Phosphatidylinositols
Immunoprecipitation
Tyrosine
Anti-Idiotypic Antibodies
Phosphotransferases
Spleen
Molecular Weight
Lung
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cohen, B., Liu, Y., Druker, B., Roberts, T. M., & Schaffhausen, B. S. (1990). Characterization of pp85, a Target of Oncogenes and Growth Factor Receptors. Molecular and Cellular Biology, 10(6), 2909-2915.

Characterization of pp85, a Target of Oncogenes and Growth Factor Receptors. / Cohen, Bruce; Liu, Yuxi; Druker, Brian; Roberts, Thomas M.; Schaffhausen, Brian S.

In: Molecular and Cellular Biology, Vol. 10, No. 6, 06.1990, p. 2909-2915.

Research output: Contribution to journalArticle

Cohen, B, Liu, Y, Druker, B, Roberts, TM & Schaffhausen, BS 1990, 'Characterization of pp85, a Target of Oncogenes and Growth Factor Receptors', Molecular and Cellular Biology, vol. 10, no. 6, pp. 2909-2915.
Cohen, Bruce ; Liu, Yuxi ; Druker, Brian ; Roberts, Thomas M. ; Schaffhausen, Brian S. / Characterization of pp85, a Target of Oncogenes and Growth Factor Receptors. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 6. pp. 2909-2915.
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