Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5

Joseph Califano, T. Kitten, J. P. Lewis, F. L. Macrina, R. D. Fleischmann, C. M. Fraser, M. J. Duncan, F. E. Dewhirst

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In contrast to many bacterial pathogens, P. gingivalis strains display considerable variability, which is likely due to genetic exchange and intragenomic changes. To explore the latter possibility, we have studied the occurrence of insertion sequence (IS)-like elements in P. gingivalis W83 by utilizing a convenient and rapid method of capturing IS-like sequences and through analysis of the genome sequence of P. gingivalis strain W83. We adapted the method of Matsutani et al. (S. Matsutani, H. Ohtsubo, Y. Maeda, and E. Ohtsubo, J. Mol. Biol. 196:445-455, 1987) to isolate and clone rapidly annealing DNA sequences characteristic of repetitive regions within a genome. We show that in P. gingivalis strain W83, such sequences include (i) nucleotide sequence with homology to tRNA genes, (ii) a previously described IS element, and (iii) a novel IS-like element. Analysis of the P. gingivalis genome sequence for the distribution of the least used tetranucleotide, CTAG, identified regions in many of the initial 218 contigs which contained CTAG clusters. Examination of these CTAG clusters led to the discovery of 11 copies of the same novel IS-like element identified by the repeated sequence capture method of Matsutani et al. This new 1,512-bp IS-like element, designated ISPg5, has features of the IS3 family of IS elements. When a recombinant plasmid containing much of ISPg5 was used in Southern analysis of several P. gingivalis strains, including clinical isolates, diversity among strains was apparent. This suggests that ISPg5 and other IS elements may contribute to strain diversity and can be used for strain fingerprinting.

Original languageEnglish (US)
Pages (from-to)5247-5253
Number of pages7
JournalInfection and Immunity
Volume68
Issue number9
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
DNA Transposable Elements
Periodontitis
Genome
Nucleic Acid Repetitive Sequences
Insertional Mutagenesis
Sequence Homology
Transfer RNA
Sequence Analysis
Plasmids
Clone Cells
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Califano, J., Kitten, T., Lewis, J. P., Macrina, F. L., Fleischmann, R. D., Fraser, C. M., ... Dewhirst, F. E. (2000). Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5. Infection and Immunity, 68(9), 5247-5253. https://doi.org/10.1128/IAI.68.9.5247-5253.2000

Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5. / Califano, Joseph; Kitten, T.; Lewis, J. P.; Macrina, F. L.; Fleischmann, R. D.; Fraser, C. M.; Duncan, M. J.; Dewhirst, F. E.

In: Infection and Immunity, Vol. 68, No. 9, 2000, p. 5247-5253.

Research output: Contribution to journalArticle

Califano, J, Kitten, T, Lewis, JP, Macrina, FL, Fleischmann, RD, Fraser, CM, Duncan, MJ & Dewhirst, FE 2000, 'Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5', Infection and Immunity, vol. 68, no. 9, pp. 5247-5253. https://doi.org/10.1128/IAI.68.9.5247-5253.2000
Califano, Joseph ; Kitten, T. ; Lewis, J. P. ; Macrina, F. L. ; Fleischmann, R. D. ; Fraser, C. M. ; Duncan, M. J. ; Dewhirst, F. E. / Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5. In: Infection and Immunity. 2000 ; Vol. 68, No. 9. pp. 5247-5253.
@article{a623990af33b4578b4bc8a9559a870e7,
title = "Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5",
abstract = "Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In contrast to many bacterial pathogens, P. gingivalis strains display considerable variability, which is likely due to genetic exchange and intragenomic changes. To explore the latter possibility, we have studied the occurrence of insertion sequence (IS)-like elements in P. gingivalis W83 by utilizing a convenient and rapid method of capturing IS-like sequences and through analysis of the genome sequence of P. gingivalis strain W83. We adapted the method of Matsutani et al. (S. Matsutani, H. Ohtsubo, Y. Maeda, and E. Ohtsubo, J. Mol. Biol. 196:445-455, 1987) to isolate and clone rapidly annealing DNA sequences characteristic of repetitive regions within a genome. We show that in P. gingivalis strain W83, such sequences include (i) nucleotide sequence with homology to tRNA genes, (ii) a previously described IS element, and (iii) a novel IS-like element. Analysis of the P. gingivalis genome sequence for the distribution of the least used tetranucleotide, CTAG, identified regions in many of the initial 218 contigs which contained CTAG clusters. Examination of these CTAG clusters led to the discovery of 11 copies of the same novel IS-like element identified by the repeated sequence capture method of Matsutani et al. This new 1,512-bp IS-like element, designated ISPg5, has features of the IS3 family of IS elements. When a recombinant plasmid containing much of ISPg5 was used in Southern analysis of several P. gingivalis strains, including clinical isolates, diversity among strains was apparent. This suggests that ISPg5 and other IS elements may contribute to strain diversity and can be used for strain fingerprinting.",
author = "Joseph Califano and T. Kitten and Lewis, {J. P.} and Macrina, {F. L.} and Fleischmann, {R. D.} and Fraser, {C. M.} and Duncan, {M. J.} and Dewhirst, {F. E.}",
year = "2000",
doi = "10.1128/IAI.68.9.5247-5253.2000",
language = "English (US)",
volume = "68",
pages = "5247--5253",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5

AU - Califano, Joseph

AU - Kitten, T.

AU - Lewis, J. P.

AU - Macrina, F. L.

AU - Fleischmann, R. D.

AU - Fraser, C. M.

AU - Duncan, M. J.

AU - Dewhirst, F. E.

PY - 2000

Y1 - 2000

N2 - Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In contrast to many bacterial pathogens, P. gingivalis strains display considerable variability, which is likely due to genetic exchange and intragenomic changes. To explore the latter possibility, we have studied the occurrence of insertion sequence (IS)-like elements in P. gingivalis W83 by utilizing a convenient and rapid method of capturing IS-like sequences and through analysis of the genome sequence of P. gingivalis strain W83. We adapted the method of Matsutani et al. (S. Matsutani, H. Ohtsubo, Y. Maeda, and E. Ohtsubo, J. Mol. Biol. 196:445-455, 1987) to isolate and clone rapidly annealing DNA sequences characteristic of repetitive regions within a genome. We show that in P. gingivalis strain W83, such sequences include (i) nucleotide sequence with homology to tRNA genes, (ii) a previously described IS element, and (iii) a novel IS-like element. Analysis of the P. gingivalis genome sequence for the distribution of the least used tetranucleotide, CTAG, identified regions in many of the initial 218 contigs which contained CTAG clusters. Examination of these CTAG clusters led to the discovery of 11 copies of the same novel IS-like element identified by the repeated sequence capture method of Matsutani et al. This new 1,512-bp IS-like element, designated ISPg5, has features of the IS3 family of IS elements. When a recombinant plasmid containing much of ISPg5 was used in Southern analysis of several P. gingivalis strains, including clinical isolates, diversity among strains was apparent. This suggests that ISPg5 and other IS elements may contribute to strain diversity and can be used for strain fingerprinting.

AB - Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In contrast to many bacterial pathogens, P. gingivalis strains display considerable variability, which is likely due to genetic exchange and intragenomic changes. To explore the latter possibility, we have studied the occurrence of insertion sequence (IS)-like elements in P. gingivalis W83 by utilizing a convenient and rapid method of capturing IS-like sequences and through analysis of the genome sequence of P. gingivalis strain W83. We adapted the method of Matsutani et al. (S. Matsutani, H. Ohtsubo, Y. Maeda, and E. Ohtsubo, J. Mol. Biol. 196:445-455, 1987) to isolate and clone rapidly annealing DNA sequences characteristic of repetitive regions within a genome. We show that in P. gingivalis strain W83, such sequences include (i) nucleotide sequence with homology to tRNA genes, (ii) a previously described IS element, and (iii) a novel IS-like element. Analysis of the P. gingivalis genome sequence for the distribution of the least used tetranucleotide, CTAG, identified regions in many of the initial 218 contigs which contained CTAG clusters. Examination of these CTAG clusters led to the discovery of 11 copies of the same novel IS-like element identified by the repeated sequence capture method of Matsutani et al. This new 1,512-bp IS-like element, designated ISPg5, has features of the IS3 family of IS elements. When a recombinant plasmid containing much of ISPg5 was used in Southern analysis of several P. gingivalis strains, including clinical isolates, diversity among strains was apparent. This suggests that ISPg5 and other IS elements may contribute to strain diversity and can be used for strain fingerprinting.

UR - http://www.scopus.com/inward/record.url?scp=0033860739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033860739&partnerID=8YFLogxK

U2 - 10.1128/IAI.68.9.5247-5253.2000

DO - 10.1128/IAI.68.9.5247-5253.2000

M3 - Article

VL - 68

SP - 5247

EP - 5253

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 9

ER -