Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography

P. O. Bagnaninchi, Y. Yang, A. El Haf, Monica Hinds, R. K. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In order to achieve functional tissue with the correct biomechanical properties it is critical to stimulate mechanically the cells. Perfusion bioreactor induces fluid shear stress that has been well characterized for two-dimensional culture where both simulation and experimental data are available. However these results can't be directly translated to tissue engineering that makes use of complex three-dimensional porous scaffold. Moreover, stimulated cells produce extensive extra-cellular matrix (ECM) that alter dramatically the micro-architecture of the constructs, changing the local flow dynamic. In this study a Fourier domain Doppler optical coherent tomography (FD-DOCT) system working at 1300nm with a bandwidth of 50nm has been used to determine the local flow rate inside different types of porous scaffolds used in tissue engineering. Local flow rates can then be linearly related, for Newtonian fluid, to the fluid shear stress occurring on the pores wall. Porous chitosan scaffolds (φ1.5mm × 3mm) with and without a central 250 um microchannel have been produced by a freeze-drying technique. This techniques allow us to determine the actual shear stress applied to the cells and to optimise the input flow rate consequently, but also to relate the change of the flow distribution to the amount of ECM production allowing the monitoring of tissue formation.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6439
DOIs
StatePublished - 2007
EventOptics in Tissue Engineering and Regenerative Medicine - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Other

OtherOptics in Tissue Engineering and Regenerative Medicine
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Fingerprint

Optical tomography
Shear stress
Flow of fluids
Flow rate
Tissue engineering
Scaffolds
Fluids
Tissue
Scaffolds (biology)
Microchannels
Bioreactors
Chitosan
Tomography
Drying
Bandwidth
Monitoring

Keywords

  • 3D porous constructs
  • Fourier domain Doppler OCT
  • Interconnectivity
  • Local fluid flow
  • Porous scaffold
  • Shear stress
  • Tissue engineering

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Bagnaninchi, P. O., Yang, Y., El Haf, A., Hinds, M., & Wang, R. K. (2007). Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6439). [64390H] https://doi.org/10.1117/12.701083

Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography. / Bagnaninchi, P. O.; Yang, Y.; El Haf, A.; Hinds, Monica; Wang, R. K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439 2007. 64390H.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bagnaninchi, PO, Yang, Y, El Haf, A, Hinds, M & Wang, RK 2007, Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6439, 64390H, Optics in Tissue Engineering and Regenerative Medicine, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.701083
Bagnaninchi PO, Yang Y, El Haf A, Hinds M, Wang RK. Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439. 2007. 64390H https://doi.org/10.1117/12.701083
Bagnaninchi, P. O. ; Yang, Y. ; El Haf, A. ; Hinds, Monica ; Wang, R. K. / Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439 2007.
@inproceedings{90ee4315cf4c4ad0ba3177a484e461c1,
title = "Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography",
abstract = "In order to achieve functional tissue with the correct biomechanical properties it is critical to stimulate mechanically the cells. Perfusion bioreactor induces fluid shear stress that has been well characterized for two-dimensional culture where both simulation and experimental data are available. However these results can't be directly translated to tissue engineering that makes use of complex three-dimensional porous scaffold. Moreover, stimulated cells produce extensive extra-cellular matrix (ECM) that alter dramatically the micro-architecture of the constructs, changing the local flow dynamic. In this study a Fourier domain Doppler optical coherent tomography (FD-DOCT) system working at 1300nm with a bandwidth of 50nm has been used to determine the local flow rate inside different types of porous scaffolds used in tissue engineering. Local flow rates can then be linearly related, for Newtonian fluid, to the fluid shear stress occurring on the pores wall. Porous chitosan scaffolds (φ1.5mm × 3mm) with and without a central 250 um microchannel have been produced by a freeze-drying technique. This techniques allow us to determine the actual shear stress applied to the cells and to optimise the input flow rate consequently, but also to relate the change of the flow distribution to the amount of ECM production allowing the monitoring of tissue formation.",
keywords = "3D porous constructs, Fourier domain Doppler OCT, Interconnectivity, Local fluid flow, Porous scaffold, Shear stress, Tissue engineering",
author = "Bagnaninchi, {P. O.} and Y. Yang and {El Haf}, A. and Monica Hinds and Wang, {R. K.}",
year = "2007",
doi = "10.1117/12.701083",
language = "English (US)",
isbn = "0819465526",
volume = "6439",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Characterization of local fluid flow in 3D porous construct characterized by fourier domain doppler optical coherence tomography

AU - Bagnaninchi, P. O.

AU - Yang, Y.

AU - El Haf, A.

AU - Hinds, Monica

AU - Wang, R. K.

PY - 2007

Y1 - 2007

N2 - In order to achieve functional tissue with the correct biomechanical properties it is critical to stimulate mechanically the cells. Perfusion bioreactor induces fluid shear stress that has been well characterized for two-dimensional culture where both simulation and experimental data are available. However these results can't be directly translated to tissue engineering that makes use of complex three-dimensional porous scaffold. Moreover, stimulated cells produce extensive extra-cellular matrix (ECM) that alter dramatically the micro-architecture of the constructs, changing the local flow dynamic. In this study a Fourier domain Doppler optical coherent tomography (FD-DOCT) system working at 1300nm with a bandwidth of 50nm has been used to determine the local flow rate inside different types of porous scaffolds used in tissue engineering. Local flow rates can then be linearly related, for Newtonian fluid, to the fluid shear stress occurring on the pores wall. Porous chitosan scaffolds (φ1.5mm × 3mm) with and without a central 250 um microchannel have been produced by a freeze-drying technique. This techniques allow us to determine the actual shear stress applied to the cells and to optimise the input flow rate consequently, but also to relate the change of the flow distribution to the amount of ECM production allowing the monitoring of tissue formation.

AB - In order to achieve functional tissue with the correct biomechanical properties it is critical to stimulate mechanically the cells. Perfusion bioreactor induces fluid shear stress that has been well characterized for two-dimensional culture where both simulation and experimental data are available. However these results can't be directly translated to tissue engineering that makes use of complex three-dimensional porous scaffold. Moreover, stimulated cells produce extensive extra-cellular matrix (ECM) that alter dramatically the micro-architecture of the constructs, changing the local flow dynamic. In this study a Fourier domain Doppler optical coherent tomography (FD-DOCT) system working at 1300nm with a bandwidth of 50nm has been used to determine the local flow rate inside different types of porous scaffolds used in tissue engineering. Local flow rates can then be linearly related, for Newtonian fluid, to the fluid shear stress occurring on the pores wall. Porous chitosan scaffolds (φ1.5mm × 3mm) with and without a central 250 um microchannel have been produced by a freeze-drying technique. This techniques allow us to determine the actual shear stress applied to the cells and to optimise the input flow rate consequently, but also to relate the change of the flow distribution to the amount of ECM production allowing the monitoring of tissue formation.

KW - 3D porous constructs

KW - Fourier domain Doppler OCT

KW - Interconnectivity

KW - Local fluid flow

KW - Porous scaffold

KW - Shear stress

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=34548262252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548262252&partnerID=8YFLogxK

U2 - 10.1117/12.701083

DO - 10.1117/12.701083

M3 - Conference contribution

AN - SCOPUS:34548262252

SN - 0819465526

SN - 9780819465528

VL - 6439

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -