Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus

Fuming Zhang, Javier Aguilera, Julie M. Beaudet, Qing Xie, Thomas F. Lerch, Omar Davulcu, Wilfredo Colón, Michael Chapman, Robert J. Linhardt

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Adeno-associated virus (AAV) is a key candidate in the development of gene therapy. In this work, we used surface plasmon resonance spectroscopy to study the interaction between AAV and heparin and other glycosaminoglycans (GAGs). Surface plasmon resonance results revealed that heparin binds to AAV with an extremely high affinity. Solution competition studies showed that binding of AAV to heparin is chain length-dependent. AAV prefers to bind full chain heparin. All sulfo groups (especially N-sulfo and 6-O-sulfo groups) on heparin are important for the AAV-heparin interaction. Higher levels of sulfo group substitution in GAGs enhance their binding affinities. Atomic force microscopy was also performed to image AAV-2 in a complex with heparin.

Original languageEnglish (US)
Pages (from-to)6275-6285
Number of pages11
JournalBiochemistry
Volume52
Issue number36
DOIs
StatePublished - Sep 10 2013

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Dependovirus
Glycosaminoglycans
Viruses
Heparin
Surface Plasmon Resonance
Surface plasmon resonance
Gene therapy
Atomic Force Microscopy
Chain length
Genetic Therapy
Atomic force microscopy
Spectrum Analysis
Substitution reactions
Spectroscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zhang, F., Aguilera, J., Beaudet, J. M., Xie, Q., Lerch, T. F., Davulcu, O., ... Linhardt, R. J. (2013). Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus. Biochemistry, 52(36), 6275-6285. https://doi.org/10.1021/bi4008676

Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus. / Zhang, Fuming; Aguilera, Javier; Beaudet, Julie M.; Xie, Qing; Lerch, Thomas F.; Davulcu, Omar; Colón, Wilfredo; Chapman, Michael; Linhardt, Robert J.

In: Biochemistry, Vol. 52, No. 36, 10.09.2013, p. 6275-6285.

Research output: Contribution to journalArticle

Zhang, F, Aguilera, J, Beaudet, JM, Xie, Q, Lerch, TF, Davulcu, O, Colón, W, Chapman, M & Linhardt, RJ 2013, 'Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus', Biochemistry, vol. 52, no. 36, pp. 6275-6285. https://doi.org/10.1021/bi4008676
Zhang F, Aguilera J, Beaudet JM, Xie Q, Lerch TF, Davulcu O et al. Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus. Biochemistry. 2013 Sep 10;52(36):6275-6285. https://doi.org/10.1021/bi4008676
Zhang, Fuming ; Aguilera, Javier ; Beaudet, Julie M. ; Xie, Qing ; Lerch, Thomas F. ; Davulcu, Omar ; Colón, Wilfredo ; Chapman, Michael ; Linhardt, Robert J. / Characterization of interactions between heparin/glycosaminoglycan and adeno-associated virus. In: Biochemistry. 2013 ; Vol. 52, No. 36. pp. 6275-6285.
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