Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells

Hwa Vivian, Cindy Tomasini-Sprenger, Abel López Bermejo, Ronald (Ron) Rosenfeld, Steven R. Plymate

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Insulin-like growth factor-binding protein-related protein-1 (IGFBP- rP1; also known as Mac25, TAF, and PSF) is a member of the IGFBP superfamily. It is a cystein-rich protein that shares structural and functional similarities with the conventional IGFBPs. In situ hybridization of prostate tissue sections Show intense IGFBP-rP1 messenger ribonucleic acid (mRNA) expression in normal stroma and glandular epithelium. There was a significant loss of detectable IGFBP-rP1 mRNA in metastatic prostate tissue. IGFBP-rP1 mRNA (Northern blots) and protein (immunoblots) were detectable in primary cultures of prostatic stromal and epithelial cells as well as in the immortalized nonmalignant prostatic human epithelial cells, P69, and in the P69 metastatic subline, M12. IGFBP-rP1 expression was not detectable in the prostatic cancer cell lines PC-3, DU145, and LNCaP. IGFBP-rP1 expression was regulated in P69 cells but not in M12 cells. Protein and mRNA expression was up-regulated by IGF-I, transforming growth factor-β, and retinoic acid. The observations that IGFBP-rP1 expression is significantly diminished in prostate tumorigenesis and is regulated in nonmalignant prostate cells suggest IGFBP-rP1 is important in normal prostatic cell growth.

Original languageEnglish (US)
Pages (from-to)4355-4362
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume83
Issue number12
StatePublished - 1998

Fingerprint

Prostate
RNA
Insulin-Like Growth Factor Binding Proteins
Epithelial Cells
Tissue
insulin-like growth factor binding protein-related protein 1
Proteins
Cell growth
Transforming Growth Factors
Stromal Cells
Tretinoin
Insulin-Like Growth Factor I
Northern Blotting
In Situ Hybridization
Prostatic Neoplasms
Carcinogenesis
Epithelium
Cells
Cell Line
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Vivian, H., Tomasini-Sprenger, C., López Bermejo, A., Rosenfeld, R. R., & Plymate, S. R. (1998). Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells. Journal of Clinical Endocrinology and Metabolism, 83(12), 4355-4362.

Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells. / Vivian, Hwa; Tomasini-Sprenger, Cindy; López Bermejo, Abel; Rosenfeld, Ronald (Ron); Plymate, Steven R.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 83, No. 12, 1998, p. 4355-4362.

Research output: Contribution to journalArticle

Vivian, H, Tomasini-Sprenger, C, López Bermejo, A, Rosenfeld, RR & Plymate, SR 1998, 'Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells', Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 12, pp. 4355-4362.
Vivian H, Tomasini-Sprenger C, López Bermejo A, Rosenfeld RR, Plymate SR. Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells. Journal of Clinical Endocrinology and Metabolism. 1998;83(12):4355-4362.
Vivian, Hwa ; Tomasini-Sprenger, Cindy ; López Bermejo, Abel ; Rosenfeld, Ronald (Ron) ; Plymate, Steven R. / Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells. In: Journal of Clinical Endocrinology and Metabolism. 1998 ; Vol. 83, No. 12. pp. 4355-4362.
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