Characterization of human platelet binding of recombinant T cell receptor ligand

Asako Itakura, Joseph Aslan, Sushmita Sinha, Tara C. White-Adams, Ishan A. Patel, Roberto Meza-Romero, Arthur Vandenbark, Gregory G. Burrows, Halina Offner, Owen McCarty

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.

Original languageEnglish (US)
Article number75
JournalJournal of Neuroinflammation
Volume7
DOIs
StatePublished - Nov 8 2010

Fingerprint

T-Cell Antigen Receptor
Blood Platelets
Ligands
Thrombosis
Collagen
T-Lymphocytes
Pseudopodia
Autoimmune Experimental Encephalomyelitis
src-Family Kinases
Major Histocompatibility Complex
Hemostasis
Phosphatidylinositol 3-Kinases
Platelet Aggregation
Dendritic Cells
Multiple Sclerosis
Immunity
Blood Cells
B-Lymphocytes
Macrophages
Inflammation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology
  • Immunology
  • Neuroscience(all)

Cite this

Characterization of human platelet binding of recombinant T cell receptor ligand. / Itakura, Asako; Aslan, Joseph; Sinha, Sushmita; White-Adams, Tara C.; Patel, Ishan A.; Meza-Romero, Roberto; Vandenbark, Arthur; Burrows, Gregory G.; Offner, Halina; McCarty, Owen.

In: Journal of Neuroinflammation, Vol. 7, 75, 08.11.2010.

Research output: Contribution to journalArticle

Itakura, Asako ; Aslan, Joseph ; Sinha, Sushmita ; White-Adams, Tara C. ; Patel, Ishan A. ; Meza-Romero, Roberto ; Vandenbark, Arthur ; Burrows, Gregory G. ; Offner, Halina ; McCarty, Owen. / Characterization of human platelet binding of recombinant T cell receptor ligand. In: Journal of Neuroinflammation. 2010 ; Vol. 7.
@article{43193a2ee0c942de9d02ae2036dea590,
title = "Characterization of human platelet binding of recombinant T cell receptor ligand",
abstract = "Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.",
author = "Asako Itakura and Joseph Aslan and Sushmita Sinha and White-Adams, {Tara C.} and Patel, {Ishan A.} and Roberto Meza-Romero and Arthur Vandenbark and Burrows, {Gregory G.} and Halina Offner and Owen McCarty",
year = "2010",
month = "11",
day = "8",
doi = "10.1186/1742-2094-7-75",
language = "English (US)",
volume = "7",
journal = "Journal of Neuroinflammation",
issn = "1742-2094",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Characterization of human platelet binding of recombinant T cell receptor ligand

AU - Itakura, Asako

AU - Aslan, Joseph

AU - Sinha, Sushmita

AU - White-Adams, Tara C.

AU - Patel, Ishan A.

AU - Meza-Romero, Roberto

AU - Vandenbark, Arthur

AU - Burrows, Gregory G.

AU - Offner, Halina

AU - McCarty, Owen

PY - 2010/11/8

Y1 - 2010/11/8

N2 - Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.

AB - Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.

UR - http://www.scopus.com/inward/record.url?scp=78049459696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049459696&partnerID=8YFLogxK

U2 - 10.1186/1742-2094-7-75

DO - 10.1186/1742-2094-7-75

M3 - Article

VL - 7

JO - Journal of Neuroinflammation

JF - Journal of Neuroinflammation

SN - 1742-2094

M1 - 75

ER -