Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays

Jun Li, Wei Zhao, Rehan Akbani, Wenbin Liu, Zhenlin Ju, Shiyun Ling, Christopher P. Vellano, Paul Roebuck, Qinghua Yu, A. Karina Eterovic, Lauren A. Byers, Michael A. Davies, Wanleng Deng, Y. N.Vashisht Gopal, Guo Chen, Erika M. von Euw, Dennis Slamon, Dylan Conklin, John V. Heymach, Adi F. GazdarJohn D. Minna, Jeffrey N. Myers, Yiling Lu, Gordon Mills, Han Liang

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.

Original languageEnglish (US)
Pages (from-to)225-239
Number of pages15
JournalCancer Cell
Volume31
Issue number2
DOIs
StatePublished - Feb 13 2017
Externally publishedYes

Fingerprint

Protein Array Analysis
Cell Line
Preclinical Drug Evaluations
Neoplasms
Proteins
Phosphoproteins
Computational Biology
Pharmaceutical Preparations
Biomedical Research
RNA
Messenger RNA
DNA

Keywords

  • biomarker
  • cancer cell lines
  • data portal
  • drug sensitivity
  • proteomics
  • reverse-phase protein array
  • signaling pathways

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Cite this

Li, J., Zhao, W., Akbani, R., Liu, W., Ju, Z., Ling, S., ... Liang, H. (2017). Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. Cancer Cell, 31(2), 225-239. https://doi.org/10.1016/j.ccell.2017.01.005

Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. / Li, Jun; Zhao, Wei; Akbani, Rehan; Liu, Wenbin; Ju, Zhenlin; Ling, Shiyun; Vellano, Christopher P.; Roebuck, Paul; Yu, Qinghua; Eterovic, A. Karina; Byers, Lauren A.; Davies, Michael A.; Deng, Wanleng; Gopal, Y. N.Vashisht; Chen, Guo; von Euw, Erika M.; Slamon, Dennis; Conklin, Dylan; Heymach, John V.; Gazdar, Adi F.; Minna, John D.; Myers, Jeffrey N.; Lu, Yiling; Mills, Gordon; Liang, Han.

In: Cancer Cell, Vol. 31, No. 2, 13.02.2017, p. 225-239.

Research output: Contribution to journalArticle

Li, J, Zhao, W, Akbani, R, Liu, W, Ju, Z, Ling, S, Vellano, CP, Roebuck, P, Yu, Q, Eterovic, AK, Byers, LA, Davies, MA, Deng, W, Gopal, YNV, Chen, G, von Euw, EM, Slamon, D, Conklin, D, Heymach, JV, Gazdar, AF, Minna, JD, Myers, JN, Lu, Y, Mills, G & Liang, H 2017, 'Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays', Cancer Cell, vol. 31, no. 2, pp. 225-239. https://doi.org/10.1016/j.ccell.2017.01.005
Li, Jun ; Zhao, Wei ; Akbani, Rehan ; Liu, Wenbin ; Ju, Zhenlin ; Ling, Shiyun ; Vellano, Christopher P. ; Roebuck, Paul ; Yu, Qinghua ; Eterovic, A. Karina ; Byers, Lauren A. ; Davies, Michael A. ; Deng, Wanleng ; Gopal, Y. N.Vashisht ; Chen, Guo ; von Euw, Erika M. ; Slamon, Dennis ; Conklin, Dylan ; Heymach, John V. ; Gazdar, Adi F. ; Minna, John D. ; Myers, Jeffrey N. ; Lu, Yiling ; Mills, Gordon ; Liang, Han. / Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. In: Cancer Cell. 2017 ; Vol. 31, No. 2. pp. 225-239.
@article{7b591e2033bb442fba9681d8562295f8,
title = "Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays",
abstract = "Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.",
keywords = "biomarker, cancer cell lines, data portal, drug sensitivity, proteomics, reverse-phase protein array, signaling pathways",
author = "Jun Li and Wei Zhao and Rehan Akbani and Wenbin Liu and Zhenlin Ju and Shiyun Ling and Vellano, {Christopher P.} and Paul Roebuck and Qinghua Yu and Eterovic, {A. Karina} and Byers, {Lauren A.} and Davies, {Michael A.} and Wanleng Deng and Gopal, {Y. N.Vashisht} and Guo Chen and {von Euw}, {Erika M.} and Dennis Slamon and Dylan Conklin and Heymach, {John V.} and Gazdar, {Adi F.} and Minna, {John D.} and Myers, {Jeffrey N.} and Yiling Lu and Gordon Mills and Han Liang",
year = "2017",
month = "2",
day = "13",
doi = "10.1016/j.ccell.2017.01.005",
language = "English (US)",
volume = "31",
pages = "225--239",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays

AU - Li, Jun

AU - Zhao, Wei

AU - Akbani, Rehan

AU - Liu, Wenbin

AU - Ju, Zhenlin

AU - Ling, Shiyun

AU - Vellano, Christopher P.

AU - Roebuck, Paul

AU - Yu, Qinghua

AU - Eterovic, A. Karina

AU - Byers, Lauren A.

AU - Davies, Michael A.

AU - Deng, Wanleng

AU - Gopal, Y. N.Vashisht

AU - Chen, Guo

AU - von Euw, Erika M.

AU - Slamon, Dennis

AU - Conklin, Dylan

AU - Heymach, John V.

AU - Gazdar, Adi F.

AU - Minna, John D.

AU - Myers, Jeffrey N.

AU - Lu, Yiling

AU - Mills, Gordon

AU - Liang, Han

PY - 2017/2/13

Y1 - 2017/2/13

N2 - Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.

AB - Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.

KW - biomarker

KW - cancer cell lines

KW - data portal

KW - drug sensitivity

KW - proteomics

KW - reverse-phase protein array

KW - signaling pathways

UR - http://www.scopus.com/inward/record.url?scp=85012908020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85012908020&partnerID=8YFLogxK

U2 - 10.1016/j.ccell.2017.01.005

DO - 10.1016/j.ccell.2017.01.005

M3 - Article

C2 - 28196595

AN - SCOPUS:85012908020

VL - 31

SP - 225

EP - 239

JO - Cancer Cell

JF - Cancer Cell

SN - 1535-6108

IS - 2

ER -