Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells

Ronald (Ron) Rosenfeld, G. Ceda, D. M. Wilson, L. A. Dollar, A. R. Hoffman

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Primary cultures of rat anterior pituitary cells were assessed for the presence of specific receptors for insulin and for the somatomedin peptides, insulin-like growth factors I and II (IGF-1 and IGF-II). Specific binding per 100,000 pituitary cells averaged 9.45 ± 1.69% (mean ± SD) for [125I]IGF-II, 0.83 ± 0.06% for [125I]IGF-I, and only 0.11% for [125I]insulin. IGF-II was twice as potent as IGF-I in displacing [125I]IGF-II, while insulin was totally nonreactive. IGF-I was 5-fold more potent than IGF-II at displacing [125I]IGF-I and 1000-fold more potent than insulin. Scatchard analysis of [125I]IGF-II binding revealed a curvilinear plot, which could be resolved into a high affinity receptor with a K(a) of 7.0 x 108 M-1 and 120,000 receptor sites/cell, and a low affinity receptor with a K(a) of 1.1 x 108 M-1 and 720,000 receptor sites/cell. The existence of abundant high affinity somatomedin receptors (especially for IGF-II) on rat anterior pituitary cells is consistent with a potential role for these peptides in the regulation of GH secretion.

Original languageEnglish (US)
Pages (from-to)1571-1575
Number of pages5
JournalEndocrinology
Volume114
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
IGF Type 1 Receptor
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Somatomedin Receptors
Insulin
Peptides
Insulin Receptor

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rosenfeld, R. R., Ceda, G., Wilson, D. M., Dollar, L. A., & Hoffman, A. R. (1984). Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells. Endocrinology, 114(5), 1571-1575.

Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells. / Rosenfeld, Ronald (Ron); Ceda, G.; Wilson, D. M.; Dollar, L. A.; Hoffman, A. R.

In: Endocrinology, Vol. 114, No. 5, 1984, p. 1571-1575.

Research output: Contribution to journalArticle

Rosenfeld, RR, Ceda, G, Wilson, DM, Dollar, LA & Hoffman, AR 1984, 'Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells', Endocrinology, vol. 114, no. 5, pp. 1571-1575.
Rosenfeld, Ronald (Ron) ; Ceda, G. ; Wilson, D. M. ; Dollar, L. A. ; Hoffman, A. R. / Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells. In: Endocrinology. 1984 ; Vol. 114, No. 5. pp. 1571-1575.
@article{8523c741c52e4fff8d59fcd3c356ab4c,
title = "Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells",
abstract = "Primary cultures of rat anterior pituitary cells were assessed for the presence of specific receptors for insulin and for the somatomedin peptides, insulin-like growth factors I and II (IGF-1 and IGF-II). Specific binding per 100,000 pituitary cells averaged 9.45 ± 1.69{\%} (mean ± SD) for [125I]IGF-II, 0.83 ± 0.06{\%} for [125I]IGF-I, and only 0.11{\%} for [125I]insulin. IGF-II was twice as potent as IGF-I in displacing [125I]IGF-II, while insulin was totally nonreactive. IGF-I was 5-fold more potent than IGF-II at displacing [125I]IGF-I and 1000-fold more potent than insulin. Scatchard analysis of [125I]IGF-II binding revealed a curvilinear plot, which could be resolved into a high affinity receptor with a K(a) of 7.0 x 108 M-1 and 120,000 receptor sites/cell, and a low affinity receptor with a K(a) of 1.1 x 108 M-1 and 720,000 receptor sites/cell. The existence of abundant high affinity somatomedin receptors (especially for IGF-II) on rat anterior pituitary cells is consistent with a potential role for these peptides in the regulation of GH secretion.",
author = "Rosenfeld, {Ronald (Ron)} and G. Ceda and Wilson, {D. M.} and Dollar, {L. A.} and Hoffman, {A. R.}",
year = "1984",
language = "English (US)",
volume = "114",
pages = "1571--1575",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells

AU - Rosenfeld, Ronald (Ron)

AU - Ceda, G.

AU - Wilson, D. M.

AU - Dollar, L. A.

AU - Hoffman, A. R.

PY - 1984

Y1 - 1984

N2 - Primary cultures of rat anterior pituitary cells were assessed for the presence of specific receptors for insulin and for the somatomedin peptides, insulin-like growth factors I and II (IGF-1 and IGF-II). Specific binding per 100,000 pituitary cells averaged 9.45 ± 1.69% (mean ± SD) for [125I]IGF-II, 0.83 ± 0.06% for [125I]IGF-I, and only 0.11% for [125I]insulin. IGF-II was twice as potent as IGF-I in displacing [125I]IGF-II, while insulin was totally nonreactive. IGF-I was 5-fold more potent than IGF-II at displacing [125I]IGF-I and 1000-fold more potent than insulin. Scatchard analysis of [125I]IGF-II binding revealed a curvilinear plot, which could be resolved into a high affinity receptor with a K(a) of 7.0 x 108 M-1 and 120,000 receptor sites/cell, and a low affinity receptor with a K(a) of 1.1 x 108 M-1 and 720,000 receptor sites/cell. The existence of abundant high affinity somatomedin receptors (especially for IGF-II) on rat anterior pituitary cells is consistent with a potential role for these peptides in the regulation of GH secretion.

AB - Primary cultures of rat anterior pituitary cells were assessed for the presence of specific receptors for insulin and for the somatomedin peptides, insulin-like growth factors I and II (IGF-1 and IGF-II). Specific binding per 100,000 pituitary cells averaged 9.45 ± 1.69% (mean ± SD) for [125I]IGF-II, 0.83 ± 0.06% for [125I]IGF-I, and only 0.11% for [125I]insulin. IGF-II was twice as potent as IGF-I in displacing [125I]IGF-II, while insulin was totally nonreactive. IGF-I was 5-fold more potent than IGF-II at displacing [125I]IGF-I and 1000-fold more potent than insulin. Scatchard analysis of [125I]IGF-II binding revealed a curvilinear plot, which could be resolved into a high affinity receptor with a K(a) of 7.0 x 108 M-1 and 120,000 receptor sites/cell, and a low affinity receptor with a K(a) of 1.1 x 108 M-1 and 720,000 receptor sites/cell. The existence of abundant high affinity somatomedin receptors (especially for IGF-II) on rat anterior pituitary cells is consistent with a potential role for these peptides in the regulation of GH secretion.

UR - http://www.scopus.com/inward/record.url?scp=0021321391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021321391&partnerID=8YFLogxK

M3 - Article

C2 - 6325123

AN - SCOPUS:0021321391

VL - 114

SP - 1571

EP - 1575

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -