Characterization of GABAergic neurons in hippocampal cell cultures

D. L. Benson, F. H. Watkins, O. Steward, G. Banker

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151 Scopus citations

Abstract

The morphological characteristics of GABAergic neurons and the distribution of GABAergic synaptic terminals were examined in cultures of hippocampal neurons from 4-35 days in vitro. Neurons expressing GABA immunoreactivity represented about 6% of the total number of cultured neurons at all time points. Although the morphological characteristics of GABAergic cells suggested a heterogeneous population, GABAergic cells as a class were notably different from the non-GABAergic, presumably pyramidal cells. Most GABAergic cells had more fusiform or polygonal shaped somata, non-spiny and less tapering dendrites and appeared more phase-dense than nonGABAergic cells. Quantitative analysis revealed that GABAergic cells had fewer primary dendrites, more elongated dendritic arbors, and longer dendritic segments than non-GABAergic neurons-characteristics that are similar to GABAergic cells in situ. Double immunostaining revealed that GAD65-positive varicosities were also immunopositive for synapsin I, suggesting that GAD65-positive varicosities that contacted somata and dendrites represented presynaptic specializations. Confocal microscopy revealed the proportion of the synaptic specializations on the cell soma that were GAD65-positive was greater than on the dendrites, suggesting that somata and dendrites differ in their ability to induce the formation of presynaptic specializations by GABAergic axons. These data indicate that the GABAergic cells that develop in culture exhibit distinctive morphological characteristics and participate in different synaptic interactions than nonGABA cells. Thus many of the features that distinguish GABAergic neurons in culture are reminiscent of the characteristics that distinguish GABAergic neurons in situ.

Original languageEnglish (US)
Pages (from-to)279-295
Number of pages17
JournalJournal of Neurocytology
Volume23
Issue number5
DOIs
StatePublished - May 1 1994

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology
  • Cell Biology

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    Benson, D. L., Watkins, F. H., Steward, O., & Banker, G. (1994). Characterization of GABAergic neurons in hippocampal cell cultures. Journal of Neurocytology, 23(5), 279-295. https://doi.org/10.1007/BF01188497