Characterization of full-length MHC class II sequences in Indonesian and Vietnamese cynomolgus macaques

Hannah M. Creager, Ericka A. Becker, Kelly K. Sandman, Julie A. Karl, Simon M. Lank, Benjamin N. Bimber, Roger W. Wiseman, Austin L. Hughes, Shelby L. O'Connor, David H. O'Connor

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

In recent years, the use of cynomolgus macaques in biomedical research has increased greatly. However, with the exception of the Mauritian population, knowledge of the MHC class II genetics of the species remains limited. Here, using cDNA cloning and Sanger sequencing, we identified 127 full-length MHC class II alleles in a group of 12 Indonesian and 12 Vietnamese cynomolgus macaques. Forty two of these were completely novel to cynomolgus macaques while 61 extended the sequence of previously identified alleles from partial to full length. This more than doubles the number of full-length cynomolgus macaque MHC class II alleles available in GenBank, significantly expanding the allele library for the species and laying the groundwork for future evolutionary and functional studies.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalImmunogenetics
Volume63
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

Keywords

  • Cynomolgus macaques
  • Immunogenetics
  • MHC
  • Macaca fascicularis

ASJC Scopus subject areas

  • Immunology
  • Genetics

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