Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin

C. T. Bond, G. Nilaver, B. Godfrey, E. A. Zimmerman, John Adelman

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cloned cDNAs encoding the precursor protein for motilin and a novel peptide, motilin-associated peptide, were isolated from a library derived from porcine intestinal mucosa mRNA. Nucleotide sequence analysis predicts a precursor protein of 119 amino acids including a signal peptide in direct linkage with the 22 amino acid sequence for motilin, and a 70 amino acid peptide of unknown function. The putative bioactive moieties are separated by Lys-Lys, dibasic residues that serve as substrates for cleavage by proteolytic maturation enzymes in many polyprotein precursors. While there is an abundant literature detailing a spectrum of tissues and cell types which express motilin like immunoactivity, analysis of mRNA derived from many of these tissues suggests that the mRNA for the mucosal motilin precursor is only transcribed in this tissue. The nature of the immunoreactive material in the central nervous system and other peripheral tissues remains to be determined.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalMolecular Endocrinology
Volume2
Issue number2
StatePublished - 1988

Fingerprint

Motilin
Swine
Protein Precursors
DNA
Messenger RNA
lysyllysine
Amino Acids
Polyproteins
Peptides
Intestinal Mucosa
Protein Sorting Signals
Libraries
Sequence Analysis
Amino Acid Sequence
Peptide Hydrolases
Central Nervous System
Complementary DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Bond, C. T., Nilaver, G., Godfrey, B., Zimmerman, E. A., & Adelman, J. (1988). Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin. Molecular Endocrinology, 2(2), 175-180.

Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin. / Bond, C. T.; Nilaver, G.; Godfrey, B.; Zimmerman, E. A.; Adelman, John.

In: Molecular Endocrinology, Vol. 2, No. 2, 1988, p. 175-180.

Research output: Contribution to journalArticle

Bond, CT, Nilaver, G, Godfrey, B, Zimmerman, EA & Adelman, J 1988, 'Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin', Molecular Endocrinology, vol. 2, no. 2, pp. 175-180.
Bond CT, Nilaver G, Godfrey B, Zimmerman EA, Adelman J. Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin. Molecular Endocrinology. 1988;2(2):175-180.
Bond, C. T. ; Nilaver, G. ; Godfrey, B. ; Zimmerman, E. A. ; Adelman, John. / Characterization of complementary deoxyribonucleic acid for precursor of porcine motilin. In: Molecular Endocrinology. 1988 ; Vol. 2, No. 2. pp. 175-180.
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