Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors

Edward H. Cho, Marco Wendel, Madelyn Luttgen, Craig Yoshioka, Dena Marrinucci, Daniel Lazar, Ethan Schram, Jorge Nieva, Lyudmila Bazhenova, Alison Morgan, Andrew H. Ko, W. Michael Korn, Anand Kolatkar, Kelly Bethel, Peter Kuhn

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43% of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.

Original languageEnglish (US)
Article number016001
JournalPhysical Biology
Volume9
Issue number1
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

Circulating Neoplastic Cells
Neoplasms
Venous Thromboembolism
Neoplasm Metastasis
Cell Aggregation
Population Characteristics
Blood Donors
Population
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Structural Biology

Cite this

Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors. / Cho, Edward H.; Wendel, Marco; Luttgen, Madelyn; Yoshioka, Craig; Marrinucci, Dena; Lazar, Daniel; Schram, Ethan; Nieva, Jorge; Bazhenova, Lyudmila; Morgan, Alison; Ko, Andrew H.; Korn, W. Michael; Kolatkar, Anand; Bethel, Kelly; Kuhn, Peter.

In: Physical Biology, Vol. 9, No. 1, 016001, 02.2012.

Research output: Contribution to journalArticle

Cho, EH, Wendel, M, Luttgen, M, Yoshioka, C, Marrinucci, D, Lazar, D, Schram, E, Nieva, J, Bazhenova, L, Morgan, A, Ko, AH, Korn, WM, Kolatkar, A, Bethel, K & Kuhn, P 2012, 'Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors', Physical Biology, vol. 9, no. 1, 016001. https://doi.org/10.1088/1478-3975/9/1/016001
Cho, Edward H. ; Wendel, Marco ; Luttgen, Madelyn ; Yoshioka, Craig ; Marrinucci, Dena ; Lazar, Daniel ; Schram, Ethan ; Nieva, Jorge ; Bazhenova, Lyudmila ; Morgan, Alison ; Ko, Andrew H. ; Korn, W. Michael ; Kolatkar, Anand ; Bethel, Kelly ; Kuhn, Peter. / Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors. In: Physical Biology. 2012 ; Vol. 9, No. 1.
@article{cbea014e27834a51897720747d3699ac,
title = "Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors",
abstract = "Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43{\%} of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.",
author = "Cho, {Edward H.} and Marco Wendel and Madelyn Luttgen and Craig Yoshioka and Dena Marrinucci and Daniel Lazar and Ethan Schram and Jorge Nieva and Lyudmila Bazhenova and Alison Morgan and Ko, {Andrew H.} and Korn, {W. Michael} and Anand Kolatkar and Kelly Bethel and Peter Kuhn",
year = "2012",
month = "2",
doi = "10.1088/1478-3975/9/1/016001",
language = "English (US)",
volume = "9",
journal = "Physical Biology",
issn = "1478-3967",
publisher = "IOP Publishing Ltd.",
number = "1",

}

TY - JOUR

T1 - Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors

AU - Cho, Edward H.

AU - Wendel, Marco

AU - Luttgen, Madelyn

AU - Yoshioka, Craig

AU - Marrinucci, Dena

AU - Lazar, Daniel

AU - Schram, Ethan

AU - Nieva, Jorge

AU - Bazhenova, Lyudmila

AU - Morgan, Alison

AU - Ko, Andrew H.

AU - Korn, W. Michael

AU - Kolatkar, Anand

AU - Bethel, Kelly

AU - Kuhn, Peter

PY - 2012/2

Y1 - 2012/2

N2 - Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43% of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.

AB - Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43% of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.

UR - http://www.scopus.com/inward/record.url?scp=84856948692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856948692&partnerID=8YFLogxK

U2 - 10.1088/1478-3975/9/1/016001

DO - 10.1088/1478-3975/9/1/016001

M3 - Article

C2 - 22306705

AN - SCOPUS:84856948692

VL - 9

JO - Physical Biology

JF - Physical Biology

SN - 1478-3967

IS - 1

M1 - 016001

ER -