Characterization of an in vitro Laser thrombolysis model

A. D. Janis, A. N. Nyara, K. W. Gregory, L. A. Buckley

Research output: Contribution to journalConference article

4 Scopus citations

Abstract

Laser thrombolysis (LT) is under investigation as a safe and rapid therapy for arterial recanalization in acute embolic stroke. Clot formation is a complex process affected by many factors that lead to differences in strength and hemoglobin concentration between samples formed from whole blood. The strength of thrombus formed in vivo also varies with age. We have developed a reproducible and clinically relevant reconstituted clot model for the in vitro assessment of a laser thrombolysis system. Laser thrombolysis experiments were performed using a 577nm 1 μsec pulsed dye laser at an energy of approximately 25mJ and a repetition rate of 4Hz. Laser ablation, tensile strength, scanning electron microscopic, and histological comparisons are made with other in vitro clot models. We have found that the tensile strength of this clot model is directly proportional to the fibrinogen concentration. Laser ablation and tensile pull test studies demonstrate that LT ablation efficiency (in μg/mJ/pulse) is not significantly affected by differences in clot tensile strength. This agrees with previous studies using dye and gelatin. Our results provide support for the effective use of this laser thrombolysis system for the removal of clots of varied age and strength.

Original languageEnglish (US)
Pages (from-to)442-453
Number of pages12
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4244
DOIs
StatePublished - Jan 1 2001
EventLaser in Surgery: Advanced Characterization, Therapeutics, and Systems XI - San Jose,CA, United States
Duration: Jun 20 2001Jun 23 2001

Keywords

  • Clot model
  • In vitro
  • Laser thrombolysis
  • Stroke

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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