Characterization of an in vitro Laser thrombolysis model

A. D. Janis, A. N. Nyara, Kenton Gregory, L. A. Buckley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Laser thrombolysis (LT) is under investigation as a safe and rapid therapy for arterial recanalization in acute embolic stroke. Clot formation is a complex process affected by many factors that lead to differences in strength and hemoglobin concentration between samples formed from whole blood. The strength of thrombus formed in vivo also varies with age. We have developed a reproducible and clinically relevant reconstituted clot model for the in vitro assessment of a laser thrombolysis system. Laser thrombolysis experiments were performed using a 577nm 1 μsec pulsed dye laser at an energy of approximately 25mJ and a repetition rate of 4Hz. Laser ablation, tensile strength, scanning electron microscopic, and histological comparisons are made with other in vitro clot models. We have found that the tensile strength of this clot model is directly proportional to the fibrinogen concentration. Laser ablation and tensile pull test studies demonstrate that LT ablation efficiency (in μg/mJ/pulse) is not significantly affected by differences in clot tensile strength. This agrees with previous studies using dye and gelatin. Our results provide support for the effective use of this laser thrombolysis system for the removal of clots of varied age and strength.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.R. Anderson, K.E. Bartels, L.S. Bass, C.G. Garrett
Pages442-453
Number of pages12
Volume4244
DOIs
StatePublished - 2001
EventLaser in Surgery: Advanced Characterization, Therapeutics, and Systems XI - San Jose,CA, United States
Duration: Jun 20 2001Jun 23 2001

Other

OtherLaser in Surgery: Advanced Characterization, Therapeutics, and Systems XI
CountryUnited States
CitySan Jose,CA
Period6/20/016/23/01

Fingerprint

Laser ablation
tensile strength
laser ablation
Tensile strength
Lasers
lasers
fibrinogen
Dye lasers
Hemoglobin
gelatins
hemoglobin
strokes
Pulsed lasers
dye lasers
blood
Laser pulses
therapy
repetition
pulsed lasers
Blood

Keywords

  • Clot model
  • In vitro
  • Laser thrombolysis
  • Stroke

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Janis, A. D., Nyara, A. N., Gregory, K., & Buckley, L. A. (2001). Characterization of an in vitro Laser thrombolysis model. In R. R. Anderson, K. E. Bartels, L. S. Bass, & C. G. Garrett (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4244, pp. 442-453) https://doi.org/10.1117/12.427833

Characterization of an in vitro Laser thrombolysis model. / Janis, A. D.; Nyara, A. N.; Gregory, Kenton; Buckley, L. A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / R.R. Anderson; K.E. Bartels; L.S. Bass; C.G. Garrett. Vol. 4244 2001. p. 442-453.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Janis, AD, Nyara, AN, Gregory, K & Buckley, LA 2001, Characterization of an in vitro Laser thrombolysis model. in RR Anderson, KE Bartels, LS Bass & CG Garrett (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4244, pp. 442-453, Laser in Surgery: Advanced Characterization, Therapeutics, and Systems XI, San Jose,CA, United States, 6/20/01. https://doi.org/10.1117/12.427833
Janis AD, Nyara AN, Gregory K, Buckley LA. Characterization of an in vitro Laser thrombolysis model. In Anderson RR, Bartels KE, Bass LS, Garrett CG, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4244. 2001. p. 442-453 https://doi.org/10.1117/12.427833
Janis, A. D. ; Nyara, A. N. ; Gregory, Kenton ; Buckley, L. A. / Characterization of an in vitro Laser thrombolysis model. Proceedings of SPIE - The International Society for Optical Engineering. editor / R.R. Anderson ; K.E. Bartels ; L.S. Bass ; C.G. Garrett. Vol. 4244 2001. pp. 442-453
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