Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum

Q. Jiang, Minghua Li, C. J. Papasian, D. Branigan, Z. G. Xiong, J. Q. Wang, X. P. Chu

Research output: Contribution to journalArticle

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Abstract

Acid-sensing ion channels (ASICs) regulate synaptic activities and play important roles in neurodegenerative diseases. They are highly expressed in the striatum, where medium spiny neurons (MSNs) are a major population. Given that the properties of ASICs in MSNs are unknown, in this study, we characterized ASICs in MSNs of the mouse striatum. A rapid drop in extracellular pH induced transient inward currents in all MSNs. The pH value for half-maximal activation was 6.25, close to that obtained in homomeric ASIC1a channels. Based on psalmotoxin 1 and zinc sensitivity, ASIC1a (70.5% of neurons) and heteromeric ASIC1a-2 channels (29.5% of neurons) appeared responsible for the acid-induced currents in MSNs. ASIC currents were diminished in MSNs from ASIC1, but not ASIC2, null mice. Furthermore, a drop in pH induced calcium influx by activating homomeric ASIC1a channels. Activation of ASICs increased the membrane excitability of MSNs and lowering extracellular Ca2+ potentiated ASIC currents. Our data suggest that the homomeric ASIC1a channel represents a majority of the ASIC isoform in MSNs. The potential function of ASICs in the striatum requires further investigation.

Original languageEnglish (US)
Pages (from-to)55-66
Number of pages12
JournalNeuroscience
Volume162
Issue number1
DOIs
StatePublished - Aug 4 2009

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Acid Sensing Ion Channels
Neurons
Neurodegenerative Diseases
Zinc
Protein Isoforms

Keywords

  • acid-sensing ion channels
  • calcium imaging
  • medium spiny neurons
  • membrane excitability
  • patch-clamp
  • striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Jiang, Q., Li, M., Papasian, C. J., Branigan, D., Xiong, Z. G., Wang, J. Q., & Chu, X. P. (2009). Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum. Neuroscience, 162(1), 55-66. https://doi.org/10.1016/j.neuroscience.2009.04.029

Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum. / Jiang, Q.; Li, Minghua; Papasian, C. J.; Branigan, D.; Xiong, Z. G.; Wang, J. Q.; Chu, X. P.

In: Neuroscience, Vol. 162, No. 1, 04.08.2009, p. 55-66.

Research output: Contribution to journalArticle

Jiang, Q, Li, M, Papasian, CJ, Branigan, D, Xiong, ZG, Wang, JQ & Chu, XP 2009, 'Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum', Neuroscience, vol. 162, no. 1, pp. 55-66. https://doi.org/10.1016/j.neuroscience.2009.04.029
Jiang, Q. ; Li, Minghua ; Papasian, C. J. ; Branigan, D. ; Xiong, Z. G. ; Wang, J. Q. ; Chu, X. P. / Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum. In: Neuroscience. 2009 ; Vol. 162, No. 1. pp. 55-66.
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