Characterization of A1, a novel hemopoietic-specific early-response gene with sequence similarity to bcl-2

E. Y. Lin, A. Orlofsky, M. S. Berger, M. B. Prystowsky

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates hemopoietic cell proliferation, differentiation, and functional activation by inducing the expression of specific genes. As part of an investigation of the regulation of gene expression by GM-CSF, we have previously identified a novel murine GM-CSF-inducible gene, A1. In this report, we present the complete nucleotide sequence of the A1 mRNA as well as a portion of the 5'flanking region, and describe the expression pattern of the gene. The results demonstrate that A1 is a hemopoietic tissue-specific gene that is expressed in several hemopoietic cell lineages, including T-helper lymphocytes, macrophages, and neutrophils. In murine bone marrow-derived macrophages, A1 gene expression is rapidly and transiently induced by GM- CSF, and the induction was independent of de novo protein synthesis. In addition to GM-CSF, a transient induction of A1 mRNA accumulation was observed in response to LPS in macrophages. This induction is not mediated by IL-1α or IL-6, neither of which stimulate A1. In the myeloid precursor cell line, 32D cl3, A1 gene expression is stably induced during granulocyte colony-stimulating factor-stimulated myeloid cell differentiation. The A1 message encodes a predicted polypeptide with an M(r) of 20,024 and no signal peptide. The peptide sequence contains a region of 80 amino acids that shows similarity to bcl-2 and to the recently described bcl-2-related gene, MCL1. These data demonstrate that A1 is a novel early-response gene whose expression is associated with a variety of stimuli and occurs in several hemopoietic cell types.

Original languageEnglish (US)
Pages (from-to)1979-1988
Number of pages10
JournalJournal of Immunology
Volume151
Issue number4
StatePublished - Jan 1 1993

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Lin, E. Y., Orlofsky, A., Berger, M. S., & Prystowsky, M. B. (1993). Characterization of A1, a novel hemopoietic-specific early-response gene with sequence similarity to bcl-2. Journal of Immunology, 151(4), 1979-1988.