Characterization of a 3H-arginine8-vasopressin binding site in the cingulate gyrus of the rat pup

P. Szot; K. M. Myers; M. Swank; D. M. Dorsa

doi:10.1016/0196-9781(89)90017-X

Characterization of a ³H-arginine⁸-vasopressin binding site in the cingulate gyrus of the rat pup

P. Szot, K. M. Myers, M. Swank, D. M. Dorsa

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Autoradiographic analysis of 1, 8, 16 and 26-day-old rat brains showed ³H-arginine⁸-vasopressin (³H-AVP) binding to the cingulate gyrus-dorsal hippocampus (CG) only in the 8-day-old rat brain. Saturation analysis of CG membranes prepared from pups (7-10 days) and adults (90 days) revealed a small but significant increase in binding site concentration in adults compared to pups. However, the K_d of the ³H-AVP binding site increased significantly with age. The K_d of ³H-AVP binding to pup CG membranes was 0.9±0.1 nM, while the adult CG was 5.7±1.0 nM. The pharmacological specificity of ³H-AVP binding sites in the pup and adult CG was similar, but differed markedly from the profile observed in adult septal membranes. The primary specificity difference between the pup CG and septum was the reduced potency of certain V₁ receptor antagonists. In competition experiments the CG binding site showed a reduced affinity for the V₁ antagonist, [d(CH₂)₅, Tyr(Me)]AVP. This reduced affinity for the V₁ antagonist was also documented autoradiographically using ³H-[d(CH₂)₅, Tyr(Me)]AVP. The data suggest that the ³H-AVP binding site expressed in the pup CG is not identical to the V₁ type receptor present in the periphery and brain of the adult rat.

Original language	English (US)
Pages (from-to)	1231-1237
Number of pages	7
Journal	Peptides
Volume	10
Issue number	6
DOIs	https://doi.org/10.1016/0196-9781(89)90017-X
State	Published - 1989
Externally published	Yes

Keywords

Cingulate gyrus
Development
Vasopressin receptors

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(89)90017-X

Cite this

@article{96ccd660ac3b49d99267945375a1738c,

title = "Characterization of a 3H-arginine8-vasopressin binding site in the cingulate gyrus of the rat pup",

abstract = "Autoradiographic analysis of 1, 8, 16 and 26-day-old rat brains showed 3H-arginine8-vasopressin (3H-AVP) binding to the cingulate gyrus-dorsal hippocampus (CG) only in the 8-day-old rat brain. Saturation analysis of CG membranes prepared from pups (7-10 days) and adults (90 days) revealed a small but significant increase in binding site concentration in adults compared to pups. However, the Kd of the 3H-AVP binding site increased significantly with age. The Kd of 3H-AVP binding to pup CG membranes was 0.9±0.1 nM, while the adult CG was 5.7±1.0 nM. The pharmacological specificity of 3H-AVP binding sites in the pup and adult CG was similar, but differed markedly from the profile observed in adult septal membranes. The primary specificity difference between the pup CG and septum was the reduced potency of certain V1 receptor antagonists. In competition experiments the CG binding site showed a reduced affinity for the V1 antagonist, [d(CH2)5, Tyr(Me)]AVP. This reduced affinity for the V1 antagonist was also documented autoradiographically using 3H-[d(CH2)5, Tyr(Me)]AVP. The data suggest that the 3H-AVP binding site expressed in the pup CG is not identical to the V1 type receptor present in the periphery and brain of the adult rat.",

keywords = "Cingulate gyrus, Development, Vasopressin receptors",

author = "P. Szot and Myers, {K. M.} and M. Swank and Dorsa, {D. M.}",

note = "Funding Information: The authors wish to thank John Breininger and Paul Jones for their technical assistance. These studies were supported by the Veterans Administrationa nd NIH Grant NS 20311. Patricia Szot is the recipient of a NIH National Research Service Award 1 F32 AGO5456-01 from the National Institute on Aging.",

year = "1989",

doi = "10.1016/0196-9781(89)90017-X",

language = "English (US)",

volume = "10",

pages = "1231--1237",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - Characterization of a 3H-arginine8-vasopressin binding site in the cingulate gyrus of the rat pup

AU - Szot, P.

AU - Myers, K. M.

AU - Swank, M.

AU - Dorsa, D. M.

N1 - Funding Information: The authors wish to thank John Breininger and Paul Jones for their technical assistance. These studies were supported by the Veterans Administrationa nd NIH Grant NS 20311. Patricia Szot is the recipient of a NIH National Research Service Award 1 F32 AGO5456-01 from the National Institute on Aging.

PY - 1989

Y1 - 1989

N2 - Autoradiographic analysis of 1, 8, 16 and 26-day-old rat brains showed 3H-arginine8-vasopressin (3H-AVP) binding to the cingulate gyrus-dorsal hippocampus (CG) only in the 8-day-old rat brain. Saturation analysis of CG membranes prepared from pups (7-10 days) and adults (90 days) revealed a small but significant increase in binding site concentration in adults compared to pups. However, the Kd of the 3H-AVP binding site increased significantly with age. The Kd of 3H-AVP binding to pup CG membranes was 0.9±0.1 nM, while the adult CG was 5.7±1.0 nM. The pharmacological specificity of 3H-AVP binding sites in the pup and adult CG was similar, but differed markedly from the profile observed in adult septal membranes. The primary specificity difference between the pup CG and septum was the reduced potency of certain V1 receptor antagonists. In competition experiments the CG binding site showed a reduced affinity for the V1 antagonist, [d(CH2)5, Tyr(Me)]AVP. This reduced affinity for the V1 antagonist was also documented autoradiographically using 3H-[d(CH2)5, Tyr(Me)]AVP. The data suggest that the 3H-AVP binding site expressed in the pup CG is not identical to the V1 type receptor present in the periphery and brain of the adult rat.

AB - Autoradiographic analysis of 1, 8, 16 and 26-day-old rat brains showed 3H-arginine8-vasopressin (3H-AVP) binding to the cingulate gyrus-dorsal hippocampus (CG) only in the 8-day-old rat brain. Saturation analysis of CG membranes prepared from pups (7-10 days) and adults (90 days) revealed a small but significant increase in binding site concentration in adults compared to pups. However, the Kd of the 3H-AVP binding site increased significantly with age. The Kd of 3H-AVP binding to pup CG membranes was 0.9±0.1 nM, while the adult CG was 5.7±1.0 nM. The pharmacological specificity of 3H-AVP binding sites in the pup and adult CG was similar, but differed markedly from the profile observed in adult septal membranes. The primary specificity difference between the pup CG and septum was the reduced potency of certain V1 receptor antagonists. In competition experiments the CG binding site showed a reduced affinity for the V1 antagonist, [d(CH2)5, Tyr(Me)]AVP. This reduced affinity for the V1 antagonist was also documented autoradiographically using 3H-[d(CH2)5, Tyr(Me)]AVP. The data suggest that the 3H-AVP binding site expressed in the pup CG is not identical to the V1 type receptor present in the periphery and brain of the adult rat.

KW - Cingulate gyrus

KW - Development

KW - Vasopressin receptors

UR - http://www.scopus.com/inward/record.url?scp=0024854954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024854954&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(89)90017-X

DO - 10.1016/0196-9781(89)90017-X

M3 - Article

C2 - 2622802

AN - SCOPUS:0024854954

SN - 0196-9781

VL - 10

SP - 1231

EP - 1237

JO - Peptides

JF - Peptides

IS - 6

ER -