Characterization of a salmon insulin-like growth factor I promoter

A. Koval, V. Kulik, S. Duguay, E. Plisetskaya, M. L. Adamo, C. T. Roberts, Charles Roberts, V. Kavsan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5' untranslated region (UTR) of approximately 450-550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5'-UTRs approximately 250, 245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.

Original languageEnglish (US)
Pages (from-to)1057-1062
Number of pages6
JournalDNA and Cell Biology
Volume13
Issue number10
StatePublished - 1994
Externally publishedYes

Fingerprint

Salmon
Transcription Initiation Site
Insulin-Like Growth Factor I
Nucleotides
5' Untranslated Regions
GC Rich Sequence
Messenger RNA
TATA Box
Reverse Transcriptase Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Koval, A., Kulik, V., Duguay, S., Plisetskaya, E., Adamo, M. L., Roberts, C. T., ... Kavsan, V. (1994). Characterization of a salmon insulin-like growth factor I promoter. DNA and Cell Biology, 13(10), 1057-1062.

Characterization of a salmon insulin-like growth factor I promoter. / Koval, A.; Kulik, V.; Duguay, S.; Plisetskaya, E.; Adamo, M. L.; Roberts, C. T.; Roberts, Charles; Kavsan, V.

In: DNA and Cell Biology, Vol. 13, No. 10, 1994, p. 1057-1062.

Research output: Contribution to journalArticle

Koval, A, Kulik, V, Duguay, S, Plisetskaya, E, Adamo, ML, Roberts, CT, Roberts, C & Kavsan, V 1994, 'Characterization of a salmon insulin-like growth factor I promoter', DNA and Cell Biology, vol. 13, no. 10, pp. 1057-1062.
Koval A, Kulik V, Duguay S, Plisetskaya E, Adamo ML, Roberts CT et al. Characterization of a salmon insulin-like growth factor I promoter. DNA and Cell Biology. 1994;13(10):1057-1062.
Koval, A. ; Kulik, V. ; Duguay, S. ; Plisetskaya, E. ; Adamo, M. L. ; Roberts, C. T. ; Roberts, Charles ; Kavsan, V. / Characterization of a salmon insulin-like growth factor I promoter. In: DNA and Cell Biology. 1994 ; Vol. 13, No. 10. pp. 1057-1062.
@article{f67543d5d8cb490c9311a830ee465109,
title = "Characterization of a salmon insulin-like growth factor I promoter",
abstract = "We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5' untranslated region (UTR) of approximately 450-550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5'-UTRs approximately 250, 245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.",
author = "A. Koval and V. Kulik and S. Duguay and E. Plisetskaya and Adamo, {M. L.} and Roberts, {C. T.} and Charles Roberts and V. Kavsan",
year = "1994",
language = "English (US)",
volume = "13",
pages = "1057--1062",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "10",

}

TY - JOUR

T1 - Characterization of a salmon insulin-like growth factor I promoter

AU - Koval, A.

AU - Kulik, V.

AU - Duguay, S.

AU - Plisetskaya, E.

AU - Adamo, M. L.

AU - Roberts, C. T.

AU - Roberts, Charles

AU - Kavsan, V.

PY - 1994

Y1 - 1994

N2 - We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5' untranslated region (UTR) of approximately 450-550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5'-UTRs approximately 250, 245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.

AB - We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5' untranslated region (UTR) of approximately 450-550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5'-UTRs approximately 250, 245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.

UR - http://www.scopus.com/inward/record.url?scp=0028050674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028050674&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 1057

EP - 1062

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 10

ER -