TY - JOUR
T1 - Characterization of a Salmon Insulin-Like Growth Factor I Promoter
AU - Koval, A.
AU - Kulik, V.
AU - Kavsan, V.
AU - Duguay, S.
AU - Plisetskaya, E.
AU - Adamo, M. L.
AU - Roberts, C. T.
AU - LeRoith, D.
AU - Koval, A.
PY - 1994/10
Y1 - 1994/10
N2 - We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5′ untranslated region (UTR) of approximately 450–550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5′-UTRs approximately 250,245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.
AB - We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5′ untranslated region (UTR) of approximately 450–550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5′-UTRs approximately 250,245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.
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U2 - 10.1089/dna.1994.13.1057
DO - 10.1089/dna.1994.13.1057
M3 - Article
C2 - 7945938
AN - SCOPUS:0028050674
SN - 1044-5498
VL - 13
SP - 1057
EP - 1062
JO - DNA and Cell Biology
JF - DNA and Cell Biology
IS - 10
ER -