Characterization of a Salmon Insulin-Like Growth Factor I Promoter

A. Koval, V. Kulik, V. Kavsan, S. Duguay, E. Plisetskaya, M. L. Adamo, C. T. Roberts, D. LeRoith, A. Koval

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    10 Scopus citations

    Abstract

    We have identified four transcription initiation sites in the salmon insulin-like growth factor I (IGF-I) gene. Use of the most upstream transcription start site generates a minor mRNA species with a 5′ untranslated region (UTR) of approximately 450–550 nucleotides, whereas transcription starting at the downstream initiation sites results in more abundant IGF-I mRNAs with 5′-UTRs approximately 250,245, and 165 nucleotides in length. No consensus TATA box-like elements are present immediately upstream of the most upstream start site identified, nor is this region particularly GC-rich. Transient expression assays, however, demonstrated orientation-dependent promoter activity in a 386-nucleotide-long fragment containing the major downstream transcription start sites. Additionally, reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated tissue and developmental stage-specific use of the various transcription start sites identified.

    Original languageEnglish (US)
    Pages (from-to)1057-1062
    Number of pages6
    JournalDNA and Cell Biology
    Volume13
    Issue number10
    DOIs
    StatePublished - Oct 1994

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    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Cell Biology

    Cite this

    Koval, A., Kulik, V., Kavsan, V., Duguay, S., Plisetskaya, E., Adamo, M. L., Roberts, C. T., LeRoith, D., & Koval, A. (1994). Characterization of a Salmon Insulin-Like Growth Factor I Promoter. DNA and Cell Biology, 13(10), 1057-1062. https://doi.org/10.1089/dna.1994.13.1057