Characterization of a rat monoclonal antibody specific for a determinant encoded by the Vβ7 gene segment. Depletion of Vβ7+ T cells in mice withg Mls-1a haplotype

C. Y. Okada, B. Holzmann, C. Guidos, E. Palmer, I. L. Weissman

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

We have generated a rat mAb, TR310, which recognizes a determinant encoded by the murine Vβ7 gene segment of the TCR. TR310 immunoprecipitates TCR from cell lysates, co-modulates with CD3, and can be used for immunofluorescence staining of T cells. By using this antibody, we found that the average percentage of Vβ7+ peripheral T cells in Mls-1b mice was 3.8%, but only 0.8% in Mls-1a mice. A similar difference was also observed in the mature TCR(hi) thymocyte subsets, suggesting that Vβ7+ T cells are deleted during intrathymic maturation in Mls-1a mice. TR310 should prove to be a valuable reagent in further studies of the TCR repertoire and the analysis of factors which alter it.

Original languageEnglish (US)
Pages (from-to)3473-3477
Number of pages5
JournalJournal of Immunology
Volume144
Issue number9
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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