Characterization of a rat monoclonal antibody specific for a determinant encoded by the Vβ7 gene segment: Depletion of Vβ7+ T cells in mice with Mls-1 a haplotype

Craig Okada, Bernhard Holzmann, Cynthia Guidos, Ed Palmer, Irving L. Weissman

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117 Citations (Scopus)

Abstract

We have generated a rat mAb, TR310, which recognizes a determinant encoded by the murine Vβ7 gene segment of the TCR. TR310 immunoprecipitates TCR from cell lysates, co-modulates with CD3, and can be used for immunofluorescence staining of T cells. By using this antibody, we found that the average percentage of Vβ7+ peripheral T cells in Mls-1b mice was 3.8%, but only 0.8% in Mls-1a mice. A similar difference was also observed in the mature TCRhi thymocyte subsets, suggesting that Vβ7+ T cells are deleted during intrathymic maturation in Mls-1a mice. TR310 should prove to be a valuable reagent in further studies of the TCR repertoire and the analysis of factors which alter it.

Original languageEnglish (US)
Pages (from-to)3473-3477
Number of pages5
JournalJournal of Immunology
Volume144
Issue number9
StatePublished - May 1 1990
Externally publishedYes

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Haplotypes
Monoclonal Antibodies
T-Lymphocytes
Genes
Thymocytes
Statistical Factor Analysis
Fluorescent Antibody Technique
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of a rat monoclonal antibody specific for a determinant encoded by the Vβ7 gene segment : Depletion of Vβ7+ T cells in mice with Mls-1 a haplotype. / Okada, Craig; Holzmann, Bernhard; Guidos, Cynthia; Palmer, Ed; Weissman, Irving L.

In: Journal of Immunology, Vol. 144, No. 9, 01.05.1990, p. 3473-3477.

Research output: Contribution to journalArticle

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