Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis

Lauren M. Hook, Brooke A. Jude, Victor S. Ter-Grigorov, Janet W. Hartley, Herbert C. Morse, Zeev Trainin, Vladimir Toder, Alexander V. Chervonsky, Tatyana V. Golovkina

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A new virus previously arose in BALB/c females mated repeatedly to C57BL/6 (B6) males and then injected with fixed, activated B6 male spleen cells (V. S. Ter-Grigorov, O. Krifuks, E. Liubashevsky, A. Nyska, Z. Trainin, and V. Toder, Nat. Med. 3:37-41, 1997). In the present study, BALB/cJ mice inoculated with virus-containing plasma from affected mice developed splenomegaly, which was caused by increased numbers of Sca-1+ Lin- hematopoietic stem cells (HSC) and their differentiated progeny. Biological and molecular analyses of a new virus revealed a mixture of murine leukemia viruses (MuLVs). These MuLVs comprised ecotropic and mink lung cell focus-forming (MCF) virus classes and are termed Rauscher-like MuLVs because they bear numerous similarities to the ecotropic and MCF viruses of the Rauscher MuLV complex but do not include a spleen focus-forming virus. The ecotropic virus component alone transferred some disease characteristics, while MCF virus alone did not. Thus, we have described a novel virus mixture, termed Rauscher-like MuLV, that causes an increase in hematopoiesis due to activation of pluripotent HSC. Experiments using mice and a protocol that replicated the pregnancy and immunization strategy of the original experiment demonstrated that endogenous BALB/c mouse ecotropic and xenotropic MuLVs are activated by these treatments. Emv1 was expressed in the spleens of multiparous mice but not in those of virgin mice, and Bxv1Ernv1-pseudotyped MuLVs were recovered following injection of fixed, activated B6 cells. Thus, multiple pregnancies and allostimuli appear to have provided the signals required for activation of and recombination among endogenous viruses and could have resulted in generation of the Rauscher-like MuLV mixture.

Original languageEnglish (US)
Pages (from-to)12112-12122
Number of pages11
JournalJournal of Virology
Volume76
Issue number23
DOIs
StatePublished - Dec 2002
Externally publishedYes

Fingerprint

Retroviridae
Murine Leukemia Viruses
hematopoiesis
Murine leukemia virus
Hematopoiesis
viruses
Viruses
mice
Mink
mink
Hematopoietic Stem Cells
Lung
lungs
Spleen Focus-Forming Viruses
Spleen
Rauscher Virus
spleen
Viral Structures
Multiple Pregnancy
Pluripotent Stem Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Hook, L. M., Jude, B. A., Ter-Grigorov, V. S., Hartley, J. W., Morse, H. C., Trainin, Z., ... Golovkina, T. V. (2002). Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis. Journal of Virology, 76(23), 12112-12122. https://doi.org/10.1128/JVI.76.23.12112-12122.2002

Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis. / Hook, Lauren M.; Jude, Brooke A.; Ter-Grigorov, Victor S.; Hartley, Janet W.; Morse, Herbert C.; Trainin, Zeev; Toder, Vladimir; Chervonsky, Alexander V.; Golovkina, Tatyana V.

In: Journal of Virology, Vol. 76, No. 23, 12.2002, p. 12112-12122.

Research output: Contribution to journalArticle

Hook, LM, Jude, BA, Ter-Grigorov, VS, Hartley, JW, Morse, HC, Trainin, Z, Toder, V, Chervonsky, AV & Golovkina, TV 2002, 'Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis', Journal of Virology, vol. 76, no. 23, pp. 12112-12122. https://doi.org/10.1128/JVI.76.23.12112-12122.2002
Hook LM, Jude BA, Ter-Grigorov VS, Hartley JW, Morse HC, Trainin Z et al. Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis. Journal of Virology. 2002 Dec;76(23):12112-12122. https://doi.org/10.1128/JVI.76.23.12112-12122.2002
Hook, Lauren M. ; Jude, Brooke A. ; Ter-Grigorov, Victor S. ; Hartley, Janet W. ; Morse, Herbert C. ; Trainin, Zeev ; Toder, Vladimir ; Chervonsky, Alexander V. ; Golovkina, Tatyana V. / Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis. In: Journal of Virology. 2002 ; Vol. 76, No. 23. pp. 12112-12122.
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