Characterization of a cis element required for packaging and replication of the human hepatitis B virus

Pei-Wen Chiang, King Song Jeng, Cheng Po Hu, Chungiviing Chang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The hepatitis B virus (HBV) is a DNA virus; however, it replicates through a pregenomic RNA intermediate. Several HBV-specific mRNAs are transcribed, but only the pregenomic RNA transcript is encapsidated. The encapsidation of the HBV genome, therefore, is a highly specific and selective process. Using mutational analyses and complementation tests, we have defined a 99-nucleotide cis element located at the pre-C/C region of the HBV genome which is essential for encapsidation and the replication. Furthermore, HBV genome truncated down to 1.5 kb and HBV mutants carrying foreign DNA inserts could still replicate in our complementation system.

Original languageEnglish (US)
Pages (from-to)701-711
Number of pages11
JournalVirology
Volume186
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

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Product Packaging
Hepatitis B virus
Genome
Genetic Complementation Test
RNA
DNA Viruses
Nucleotides
Messenger RNA
DNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Characterization of a cis element required for packaging and replication of the human hepatitis B virus. / Chiang, Pei-Wen; Jeng, King Song; Hu, Cheng Po; Chang, Chungiviing.

In: Virology, Vol. 186, No. 2, 1992, p. 701-711.

Research output: Contribution to journalArticle

Chiang, Pei-Wen ; Jeng, King Song ; Hu, Cheng Po ; Chang, Chungiviing. / Characterization of a cis element required for packaging and replication of the human hepatitis B virus. In: Virology. 1992 ; Vol. 186, No. 2. pp. 701-711.
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