Characterization of a cis element required for packaging and replication of the human hepatitis B virus

Pei Wen Chiang; King Song Jeng; Cheng Po Hu; Chungiviing Chang

doi:10.1016/0042-6822(92)90037-P

Characterization of a cis element required for packaging and replication of the human hepatitis B virus

Pei Wen Chiang, King Song Jeng, Cheng Po Hu, Chungiviing Chang

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The hepatitis B virus (HBV) is a DNA virus; however, it replicates through a pregenomic RNA intermediate. Several HBV-specific mRNAs are transcribed, but only the pregenomic RNA transcript is encapsidated. The encapsidation of the HBV genome, therefore, is a highly specific and selective process. Using mutational analyses and complementation tests, we have defined a 99-nucleotide cis element located at the pre-C/C region of the HBV genome which is essential for encapsidation and the replication. Furthermore, HBV genome truncated down to 1.5 kb and HBV mutants carrying foreign DNA inserts could still replicate in our complementation system.

Original language	English (US)
Pages (from-to)	701-711
Number of pages	11
Journal	Virology
Volume	186
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(92)90037-P
State	Published - Feb 1992
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(92)90037-P

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title = "Characterization of a cis element required for packaging and replication of the human hepatitis B virus",

abstract = "The hepatitis B virus (HBV) is a DNA virus; however, it replicates through a pregenomic RNA intermediate. Several HBV-specific mRNAs are transcribed, but only the pregenomic RNA transcript is encapsidated. The encapsidation of the HBV genome, therefore, is a highly specific and selective process. Using mutational analyses and complementation tests, we have defined a 99-nucleotide cis element located at the pre-C/C region of the HBV genome which is essential for encapsidation and the replication. Furthermore, HBV genome truncated down to 1.5 kb and HBV mutants carrying foreign DNA inserts could still replicate in our complementation system.",

author = "Chiang, {Pei Wen} and Jeng, {King Song} and Hu, {Cheng Po} and Chungiviing Chang",

note = "Funding Information: We thank Professor Heinz Schaller mids pMH9/3091 and pMH3/3097, the manuscript, and Ms. Shiang-lien script. This work was supported from the National Science Council, for providing us with the plas-Dr. Kong-Bung Choofor reading Lu for preparation of the manu-by Grant NSC79-0419-8010-55 Republic of China.",

year = "1992",

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doi = "10.1016/0042-6822(92)90037-P",

language = "English (US)",

volume = "186",

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journal = "Virology",

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AU - Chiang, Pei Wen

AU - Jeng, King Song

AU - Hu, Cheng Po

AU - Chang, Chungiviing

N1 - Funding Information: We thank Professor Heinz Schaller mids pMH9/3091 and pMH3/3097, the manuscript, and Ms. Shiang-lien script. This work was supported from the National Science Council, for providing us with the plas-Dr. Kong-Bung Choofor reading Lu for preparation of the manu-by Grant NSC79-0419-8010-55 Republic of China.

PY - 1992/2

Y1 - 1992/2

N2 - The hepatitis B virus (HBV) is a DNA virus; however, it replicates through a pregenomic RNA intermediate. Several HBV-specific mRNAs are transcribed, but only the pregenomic RNA transcript is encapsidated. The encapsidation of the HBV genome, therefore, is a highly specific and selective process. Using mutational analyses and complementation tests, we have defined a 99-nucleotide cis element located at the pre-C/C region of the HBV genome which is essential for encapsidation and the replication. Furthermore, HBV genome truncated down to 1.5 kb and HBV mutants carrying foreign DNA inserts could still replicate in our complementation system.

AB - The hepatitis B virus (HBV) is a DNA virus; however, it replicates through a pregenomic RNA intermediate. Several HBV-specific mRNAs are transcribed, but only the pregenomic RNA transcript is encapsidated. The encapsidation of the HBV genome, therefore, is a highly specific and selective process. Using mutational analyses and complementation tests, we have defined a 99-nucleotide cis element located at the pre-C/C region of the HBV genome which is essential for encapsidation and the replication. Furthermore, HBV genome truncated down to 1.5 kb and HBV mutants carrying foreign DNA inserts could still replicate in our complementation system.

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Characterization of a cis element required for packaging and replication of the human hepatitis B virus

Abstract

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