TY - JOUR
T1 - Characterization of a CD4-Expressing Macaque Cell Line That Can Detect Virus after a Single Replication Cycle and Can Be Infected by Diverse Simian Immunodeficiency Virus Isolates
AU - Chackerian, Bryce
AU - Haigwood, Nancy L.
AU - Overbaugh, Julie
N1 - Funding Information:
We thank Michael Emerman for many helpful suggestions and discussion, and for comments on the manuscript. We thank Bill Sutton and Lyle Rudensey for assistance with neutralization assays, and Paul Lewis and Vanessa Hirsch for additional comments on the manuscript. We also thank J. Victor Garcia for constructing the PA317/LT4SN cell line, Adam Geballe and Bob Harrington for providing the plasmid pEQ447, Mary Tomas for assistance with FACS analysis, and Lyle Rudensey, Jason Kimata, Michael Agy, Marta Marthas, Paul Luciw, Jan McClure, and Jan North for providing virus isolates. HIV-2 CBL-20/H9, HIV-2 D194/HUT78, p239SpE3′, p239SpSp5′, and the CEM ⨯ 174 cell line were obtained through the AIDS Research and Reference Reagent Program, AIDS Program, NIAID. This work was supported by the NIH (ROI AI34251).
PY - 1995/11
Y1 - 1995/11
N2 - Primate lentiviruses such as human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) are phenotypically diverse, and virus isolates vary in cytopathicity, replication rate, and cell tropism. While all virus isolates infect primary peripheral blood lymphocytes, only a subset of strains infect established CD4-expressing T-cell lines. Here, we describe the development and characterization of a macaque cell line that can be infected by all of the strains of SIV that we have tested, including macrophage- and T-cell-tropic strains, primary and cell-line adapted strains, and SIVmac, SIVMne, and SIVsm isolates. The cells can be infected by strains of HIV type 2 (HIV-2) to varying degrees, but not by either cloned or primary isolates of HIV type 1 (HIV-1). This cell line is a derivative of a rhesus macaque mammary tumor cell line (CMMT) engineered to express human CD4. For these studies, a CMMT-CD4 clone expressing an integrated copy of a truncated HIV-1 long terminal repeat fused to theβ-galactosidasegene (LTR-β-gal) was established to allow detection of infectious SIV after a single round of replication. Here, we demonstrate the ability of the CMMT-CD4-LTR-β-galcell line to rapidly and quantitatively detect infectious SIV. Using these cells to assay virus, we could readily measure neutralizing antibody activity in animals infected with different SIV isolates. Neutralizing activity was detected against the homologous virus and lower, but detectable, activity was measured against heterologous virus. Thus, this system, which is highly sensitive and can detect infection by all of the SIV isolates we tested, is a rapid method for detecting infectious virus and quantitating neutralizing antibody activity.
AB - Primate lentiviruses such as human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) are phenotypically diverse, and virus isolates vary in cytopathicity, replication rate, and cell tropism. While all virus isolates infect primary peripheral blood lymphocytes, only a subset of strains infect established CD4-expressing T-cell lines. Here, we describe the development and characterization of a macaque cell line that can be infected by all of the strains of SIV that we have tested, including macrophage- and T-cell-tropic strains, primary and cell-line adapted strains, and SIVmac, SIVMne, and SIVsm isolates. The cells can be infected by strains of HIV type 2 (HIV-2) to varying degrees, but not by either cloned or primary isolates of HIV type 1 (HIV-1). This cell line is a derivative of a rhesus macaque mammary tumor cell line (CMMT) engineered to express human CD4. For these studies, a CMMT-CD4 clone expressing an integrated copy of a truncated HIV-1 long terminal repeat fused to theβ-galactosidasegene (LTR-β-gal) was established to allow detection of infectious SIV after a single round of replication. Here, we demonstrate the ability of the CMMT-CD4-LTR-β-galcell line to rapidly and quantitatively detect infectious SIV. Using these cells to assay virus, we could readily measure neutralizing antibody activity in animals infected with different SIV isolates. Neutralizing activity was detected against the homologous virus and lower, but detectable, activity was measured against heterologous virus. Thus, this system, which is highly sensitive and can detect infection by all of the SIV isolates we tested, is a rapid method for detecting infectious virus and quantitating neutralizing antibody activity.
UR - http://www.scopus.com/inward/record.url?scp=0028839933&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028839933&partnerID=8YFLogxK
U2 - 10.1006/viro.1995.0011
DO - 10.1006/viro.1995.0011
M3 - Article
C2 - 7491763
AN - SCOPUS:0028839933
SN - 0042-6822
VL - 213
SP - 386
EP - 394
JO - Virology
JF - Virology
IS - 2
M1 - 70011
ER -