Characterization of α2-macroglobulin from plasma of cystic fibrosis patients and controls

Stephen Back, Jack A. Alhadef

Research output: Contribution to journalArticle

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Abstract

The physical, kinetic, and isoelectric focusing properties of native α2-macroglobulin from cystic fibrosis and control plasmas were studied. No differences were found in the esterolytic activity levels of control, obligate heterozygote, and cystic fibrosis plasmas. Stability studies indicated that both control and cystic fibrosis α2-macroglobulin retained full activity for at least 8 months at -20°C, a week at 0-4°C, 11 hr at 50°C, and showed no differences in thermostability at several preincubation temperatures. The microheterogeneity of native α2-macroglobulin was studied by column isoelectric focusing of five control and five cystic fibrosis plasmas. The number and pI values of the isoelectric forms between pH 4.5-8.0 were quite similar for both groups even though consistently less cystic fibrosis α2-macroglobulin activity was recovered after isoelectric focusing.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalBiochemical Medicine
Volume30
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

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Macroglobulins
Cystic Fibrosis
Plasmas
Isoelectric Focusing
Heterozygote
Kinetics
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of α2-macroglobulin from plasma of cystic fibrosis patients and controls. / Back, Stephen; Alhadef, Jack A.

In: Biochemical Medicine, Vol. 30, No. 1, 1983, p. 34-42.

Research output: Contribution to journalArticle

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