Characterization and variation of a human inwardly-rectifying K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit

Hiroshi Sakura, Chris Bond, Margaret Warren-Perry, Sharon Horsley, Lyndal Kearney, Stephen Tucker, John Adelman, Robert Turner, Frances M. Ashcroft

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The ATP-sensitive K-channel plays a central role in insulin release from pancreatic β-cells. We report here the cloning of the gene (KCNJ6) encoding a putative subunit of a human ATP-sensitive K-channel expressed in brain and β-cells, and characterisation of its exon-intron structure. Screening of a somatic cell mapping panel and fluorescent in situ hybridization place the gene on chromosome 21 (21q22.1-22.2). Analysis of single-stranded conformational polymorphisms revealed the presence of two silent polymorphisms (Pro-149: CCG-CCA and Asp328: GAC-GAT) with similar frequencies in normal and non-insulin-dependent diabetic patients.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalFEBS Letters
Volume367
Issue number2
DOIs
StatePublished - Jun 26 1995

Fingerprint

Inwardly Rectifying Potassium Channel
Polymorphism
Genes
Adenosine Triphosphate
Gene encoding
Cloning
Chromosomes
Introns
Single-Stranded Conformational Polymorphism
Exons
Chromosomes, Human, Pair 21
Brain
Screening
Insulin
Fluorescence In Situ Hybridization
Organism Cloning
GAT

Keywords

  • ATP-sensitive K-channel
  • BIR1
  • Diabetes
  • KCNJ6
  • Pancreatic β-cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Sakura, H., Bond, C., Warren-Perry, M., Horsley, S., Kearney, L., Tucker, S., ... Ashcroft, F. M. (1995). Characterization and variation of a human inwardly-rectifying K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit. FEBS Letters, 367(2), 193-197. https://doi.org/10.1016/0014-5793(95)00498-X

Characterization and variation of a human inwardly-rectifying K-channel gene (KCNJ6) : a putative ATP-sensitive K-channel subunit. / Sakura, Hiroshi; Bond, Chris; Warren-Perry, Margaret; Horsley, Sharon; Kearney, Lyndal; Tucker, Stephen; Adelman, John; Turner, Robert; Ashcroft, Frances M.

In: FEBS Letters, Vol. 367, No. 2, 26.06.1995, p. 193-197.

Research output: Contribution to journalArticle

Sakura, H, Bond, C, Warren-Perry, M, Horsley, S, Kearney, L, Tucker, S, Adelman, J, Turner, R & Ashcroft, FM 1995, 'Characterization and variation of a human inwardly-rectifying K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit', FEBS Letters, vol. 367, no. 2, pp. 193-197. https://doi.org/10.1016/0014-5793(95)00498-X
Sakura, Hiroshi ; Bond, Chris ; Warren-Perry, Margaret ; Horsley, Sharon ; Kearney, Lyndal ; Tucker, Stephen ; Adelman, John ; Turner, Robert ; Ashcroft, Frances M. / Characterization and variation of a human inwardly-rectifying K-channel gene (KCNJ6) : a putative ATP-sensitive K-channel subunit. In: FEBS Letters. 1995 ; Vol. 367, No. 2. pp. 193-197.
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