Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n

Bernhard Weber, Colin Collins, Carolyn Robbins, R. Ellen Magenis, Allen D. Delaney, Joe Gray, Michael R. Hayden

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We present a strategy for the cloning of DNA sequences adjacent to the tandemly repeated DNA sequence (TTAGGG)n. Sequence analysis of 14 independently isolated clones revealed the presence of non-repetitive sequences immediately adjacent to or flanked by blocks of the simple repeat (TTAGGG)n. In addition, we provide sequence information on two previously undescribed tandemly repeated sequences, including a 9 bp repeat and a modification of the (TTAGGG)n repeat. Using different mapping approaches six sub-clones, free of the TTAGGG repeat, were assigned to a single human chromosome. Moreover, in situ hybridization mapped one of these subclones, G2 - 1H, definitively to the telomeric band on chromosome 4q. However, Bal 31 insensitivity suggests a location in a more subterminal region. All the (TTAGGG)n-adjacent unique sequences tested are highly conserved among primates but are not present in other mammalian species. Identification and mapping of TTAGGG-adjacent sequences will provide a refined insight into the genomic organization of the (TTAGGG)n repeat. The isolation of chromosome specific TTAGGG-adjacent sequences from subtelomeric regions of all human chromosomes will serve as important end points for the genetic maps and will be useful for the molecular characterization of chromosomal rearrangements involving telomeres.

Original languageEnglish (US)
Pages (from-to)3353-3361
Number of pages9
JournalNucleic Acids Research
Volume18
Issue number11
StatePublished - Jun 11 1990
Externally publishedYes

Fingerprint

DNA sequences
Telomere
Human Chromosomes
Chromosomes
DNA Sequence
Adjacent
Clone Cells
Chromosome
Primates
In Situ Hybridization
Sequence Analysis
Organism Cloning
Clone
Cloning
Insensitivity
End point
Rearrangement
Isolation
Genomics
Immediately

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Weber, B., Collins, C., Robbins, C., Magenis, R. E., Delaney, A. D., Gray, J., & Hayden, M. R. (1990). Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n. Nucleic Acids Research, 18(11), 3353-3361.

Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n. / Weber, Bernhard; Collins, Colin; Robbins, Carolyn; Magenis, R. Ellen; Delaney, Allen D.; Gray, Joe; Hayden, Michael R.

In: Nucleic Acids Research, Vol. 18, No. 11, 11.06.1990, p. 3353-3361.

Research output: Contribution to journalArticle

Weber, B, Collins, C, Robbins, C, Magenis, RE, Delaney, AD, Gray, J & Hayden, MR 1990, 'Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n', Nucleic Acids Research, vol. 18, no. 11, pp. 3353-3361.
Weber B, Collins C, Robbins C, Magenis RE, Delaney AD, Gray J et al. Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n. Nucleic Acids Research. 1990 Jun 11;18(11):3353-3361.
Weber, Bernhard ; Collins, Colin ; Robbins, Carolyn ; Magenis, R. Ellen ; Delaney, Allen D. ; Gray, Joe ; Hayden, Michael R. / Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 11. pp. 3353-3361.
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