Characterization and localization of 3H-arginine8-vasopressin binding to rat kidney and brain tissue

Daniel Dorsa, Lisa A. Majumdar, Frances M. Petracca, Denis G. Baskin, Lawrence E. Cornett

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Anatomic, behavioral and pharmacologic evidence suggests that arginine8-vasopressin (AVP) serves as a CNS neurotransmitter or neuromodulator. We have characterized AVP binding to membrane and tissue slice preparations from brain and kidney, and examined the anatomical distribution of these binding sites. Conditions for the binding assay were optimized using kidney medullary tissue. Binding of 3H-AVP (S.A.=30-51 Ci/mmol, NEN) to brain and kidney membranes and tissue slices was saturable, temperature dependent, linearly related to protein concentration (or number of tissue slices), reversible, and specific since the ability of cold AVP to displace 3H-AVP from binding was greater than oxytocin and other related peptide fragments. Autoradiographic localization of 3H-AVP binding was restricted to kidney medullary tissue. In brain tissue, 3H-AVP binding was found to occur in concentrated foci. Brainstem areas such as the nucleus tractus solitarius (NTS) showed a high density of AVP binding sites. Since local injections of AVP into the NTS have been shown to influence blood pressure, the present study presents the first anatomical evidence for the presence of AVP specific binding sites which might mediate this effect.

Original languageEnglish (US)
Pages (from-to)699-706
Number of pages8
JournalPeptides
Volume4
Issue number5
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Vasopressins
Rats
Brain
Tissue
Kidney
Solitary Nucleus
Binding Sites
Neurotransmitter Agents
Membranes
Peptide Fragments
Blood pressure
Oxytocin
Brain Stem
Assays
Blood Pressure
Injections
Temperature

Keywords

  • Autoradiography
  • Brain
  • Kidney
  • Neuropeptide
  • Receptors
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Characterization and localization of 3H-arginine8-vasopressin binding to rat kidney and brain tissue. / Dorsa, Daniel; Majumdar, Lisa A.; Petracca, Frances M.; Baskin, Denis G.; Cornett, Lawrence E.

In: Peptides, Vol. 4, No. 5, 1983, p. 699-706.

Research output: Contribution to journalArticle

Dorsa, Daniel ; Majumdar, Lisa A. ; Petracca, Frances M. ; Baskin, Denis G. ; Cornett, Lawrence E. / Characterization and localization of 3H-arginine8-vasopressin binding to rat kidney and brain tissue. In: Peptides. 1983 ; Vol. 4, No. 5. pp. 699-706.
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