Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel

James Douglass, Peregrine B. Osborne, Yun Cai Cai, Miles Wilkinson, Macdonald J. Christie, John Adelman

Research output: Contribution to journalArticle

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Abstract

Low stringency hybridization screening of a rat genomic DNA library with a previously described cDNA clone encoding a rat voltage-gated potassium channel has resulted in the characterization of a member of the potassium channel family, RGK5. An uninterrupted nucleotide sequence encodes a protein 525 amino acids in length, revealing that the entire coding region resides on a single exon. RGK5 transcripts are present in both mouse thymus and rat brain, as determined by Northern blot analysis. RNA transcribed in vitro from RGK5 genomic DNA directs the expression of functional potassium currents after injection into Xenopus oocytes. The currents are activated by depolarization, being half-activated at -14 mV, and inactivate almost completely during depolarizations of 1 to 2 s. The properties of the currents strongly resemble those of the type n potassium channel present on both immature thymocytes and Th lymphocytes.

Original languageEnglish (US)
Pages (from-to)4841-4850
Number of pages10
JournalJournal of Immunology
Volume144
Issue number12
StatePublished - Jun 15 1990

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Potassium Channels
Clone Cells
Lymphocytes
DNA
Voltage-Gated Potassium Channels
Genomic Library
Thymocytes
Xenopus
Gene Library
Northern Blotting
Thymus Gland
Oocytes
Exons
Potassium
Complementary DNA
RNA
Amino Acids
Injections
Brain
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Douglass, J., Osborne, P. B., Cai, Y. C., Wilkinson, M., Christie, M. J., & Adelman, J. (1990). Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. Journal of Immunology, 144(12), 4841-4850.

Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. / Douglass, James; Osborne, Peregrine B.; Cai, Yun Cai; Wilkinson, Miles; Christie, Macdonald J.; Adelman, John.

In: Journal of Immunology, Vol. 144, No. 12, 15.06.1990, p. 4841-4850.

Research output: Contribution to journalArticle

Douglass, J, Osborne, PB, Cai, YC, Wilkinson, M, Christie, MJ & Adelman, J 1990, 'Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel', Journal of Immunology, vol. 144, no. 12, pp. 4841-4850.
Douglass J, Osborne PB, Cai YC, Wilkinson M, Christie MJ, Adelman J. Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. Journal of Immunology. 1990 Jun 15;144(12):4841-4850.
Douglass, James ; Osborne, Peregrine B. ; Cai, Yun Cai ; Wilkinson, Miles ; Christie, Macdonald J. ; Adelman, John. / Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. In: Journal of Immunology. 1990 ; Vol. 144, No. 12. pp. 4841-4850.
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