TY - JOUR
T1 - Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel
AU - Douglass, James
AU - Osborne, Peregrine B.
AU - Cai, Yun Cai
AU - Wilkinson, Miles
AU - Christie, Macdonald J.
AU - Adelman, John P.
PY - 1990/6/15
Y1 - 1990/6/15
N2 - Low stringency hybridization screening of a rat genomic DNA library with a previously described cDNA clone encoding a rat voltage-gated potassium channel has resulted in the characterization of a member of the potassium channel family, RGK5. An uninterrupted nucleotide sequence encodes a protein 525 amino acids in length, revealing that the entire coding region resides on a single exon. RGK5 transcripts are present in both mouse thymus and rat brain, as determined by Northern blot analysis. RNA transcribed in vitro from RGK5 genomic DNA directs the expression of functional potassium currents after injection into Xenopus oocytes. The currents are activated by depolarization, being half-activated at -14 mV, and inactivate almost completely during depolarizations of 1 to 2 s. The properties of the currents strongly resemble those of the type n potassium channel present on both immature thymocytes and Th lymphocytes.
AB - Low stringency hybridization screening of a rat genomic DNA library with a previously described cDNA clone encoding a rat voltage-gated potassium channel has resulted in the characterization of a member of the potassium channel family, RGK5. An uninterrupted nucleotide sequence encodes a protein 525 amino acids in length, revealing that the entire coding region resides on a single exon. RGK5 transcripts are present in both mouse thymus and rat brain, as determined by Northern blot analysis. RNA transcribed in vitro from RGK5 genomic DNA directs the expression of functional potassium currents after injection into Xenopus oocytes. The currents are activated by depolarization, being half-activated at -14 mV, and inactivate almost completely during depolarizations of 1 to 2 s. The properties of the currents strongly resemble those of the type n potassium channel present on both immature thymocytes and Th lymphocytes.
UR - http://www.scopus.com/inward/record.url?scp=0025371937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025371937&partnerID=8YFLogxK
M3 - Article
C2 - 2351830
AN - SCOPUS:0025371937
SN - 0022-1767
VL - 144
SP - 4841
EP - 4850
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -