Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.

P. M. Snow, K. Zinn, Allan Harrelson, L. McAllister, J. Schilling, M. J. Bastiani, G. Makk, C. S. Goodman

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Abstract

Monoclonal antibodies were previously used to identify two glycoproteins, called fasciclin I and II (70 and 95 kDa, respectively), which are expressed on different subsets of axon fascicles in the grasshopper (Schistocerca americana) embryo. Here the monoclonal antibodies were used to purify these two membrane-associated glycoproteins for further characterization. Fasciclin II appears to be an integral membrane protein, whereas fasciclin I is an extrinsic membrane protein. The amino acid sequences of the amino terminus and fragments of both proteins were determined. Using synthetic oligonucleotide probes and antibody screening, we isolated genomic and cDNA clones. Partial DNA sequences of these clones indicate that they encode fasciclins I and II.

Original languageEnglish (US)
Pages (from-to)5291-5295
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number14
Publication statusPublished - Jul 1988
Externally publishedYes

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ASJC Scopus subject areas

  • General
  • Genetics

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