Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.

P. M. Snow, K. Zinn, A. L. Harrelson, L. McAllister, J. Schilling, M. J. Bastiani, G. Makk, C. S. Goodman

    Research output: Contribution to journalArticle

    60 Scopus citations

    Abstract

    Monoclonal antibodies were previously used to identify two glycoproteins, called fasciclin I and II (70 and 95 kDa, respectively), which are expressed on different subsets of axon fascicles in the grasshopper (Schistocerca americana) embryo. Here the monoclonal antibodies were used to purify these two membrane-associated glycoproteins for further characterization. Fasciclin II appears to be an integral membrane protein, whereas fasciclin I is an extrinsic membrane protein. The amino acid sequences of the amino terminus and fragments of both proteins were determined. Using synthetic oligonucleotide probes and antibody screening, we isolated genomic and cDNA clones. Partial DNA sequences of these clones indicate that they encode fasciclins I and II.

    Original languageEnglish (US)
    Pages (from-to)5291-5295
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume85
    Issue number14
    DOIs
    StatePublished - Jul 1988

    ASJC Scopus subject areas

    • General

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