Characteristics of phospholipid methylation in human erythrocyte ghosts

Relationship(s) to the psychoses and affective disorders

Robert Hitzemann, Cynthia Mark, Jack Hirschowitz, David Garver

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Recent studies have shown that patients with a schizophrenic-like illness have a significant deficit in erythrocyte ghost membrane (EGM) phosphatidylcholine (PC); patients with the most severe deficiency showed a marked decrease in Na+-Li+ counterflow activity (Hitzemann et al. 1984a and b). The present study was undertaken to see if the decrement in PC is associated with a decrease in phospholipid methylation activity. Phospholipid methylation in human EGMs is distinctly different from that in rat EGMs (Hirata and Axelrod 1980) in that the human activity is not Mg++-dependent, and apparent methyltransferase I activity is located in the external membrane surface. The patient population consisted of 20 DSM-III schizophrenics (SCZ), 13 DSM-III schizophreniform (SF) disorder patients, and 11 DSM-III manics (M). Twelve age- and sex-matched controls were used for the comparison group. Methylation activity was significantly decreased in all three patient groups, although the M group had significantly higher activity than the SF group. Twenty-four of the SCZ and SF patients entered a Li+ trial. The Li+ responder group (n = 8) showed significantly lower activity than the nonresponder group (n = 16). Overall, we conclude that the decrement in phospholipid methylation activity partially contributes to the decrement in PC levels.

Original languageEnglish (US)
Pages (from-to)397-407
Number of pages11
JournalBiological Psychiatry
Volume20
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Erythrocyte Membrane
Mood Disorders
Psychotic Disorders
Methylation
Phospholipids
Phosphatidylcholines
Diagnostic and Statistical Manual of Mental Disorders
Methyltransferases
Human Activities
Membranes
Population

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Characteristics of phospholipid methylation in human erythrocyte ghosts : Relationship(s) to the psychoses and affective disorders. / Hitzemann, Robert; Mark, Cynthia; Hirschowitz, Jack; Garver, David.

In: Biological Psychiatry, Vol. 20, No. 4, 1985, p. 397-407.

Research output: Contribution to journalArticle

@article{94902b9b38bd43c0adbed86d5099cf94,
title = "Characteristics of phospholipid methylation in human erythrocyte ghosts: Relationship(s) to the psychoses and affective disorders",
abstract = "Recent studies have shown that patients with a schizophrenic-like illness have a significant deficit in erythrocyte ghost membrane (EGM) phosphatidylcholine (PC); patients with the most severe deficiency showed a marked decrease in Na+-Li+ counterflow activity (Hitzemann et al. 1984a and b). The present study was undertaken to see if the decrement in PC is associated with a decrease in phospholipid methylation activity. Phospholipid methylation in human EGMs is distinctly different from that in rat EGMs (Hirata and Axelrod 1980) in that the human activity is not Mg++-dependent, and apparent methyltransferase I activity is located in the external membrane surface. The patient population consisted of 20 DSM-III schizophrenics (SCZ), 13 DSM-III schizophreniform (SF) disorder patients, and 11 DSM-III manics (M). Twelve age- and sex-matched controls were used for the comparison group. Methylation activity was significantly decreased in all three patient groups, although the M group had significantly higher activity than the SF group. Twenty-four of the SCZ and SF patients entered a Li+ trial. The Li+ responder group (n = 8) showed significantly lower activity than the nonresponder group (n = 16). Overall, we conclude that the decrement in phospholipid methylation activity partially contributes to the decrement in PC levels.",
author = "Robert Hitzemann and Cynthia Mark and Jack Hirschowitz and David Garver",
year = "1985",
doi = "10.1016/0006-3223(85)90042-3",
language = "English (US)",
volume = "20",
pages = "397--407",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier USA",
number = "4",

}

TY - JOUR

T1 - Characteristics of phospholipid methylation in human erythrocyte ghosts

T2 - Relationship(s) to the psychoses and affective disorders

AU - Hitzemann, Robert

AU - Mark, Cynthia

AU - Hirschowitz, Jack

AU - Garver, David

PY - 1985

Y1 - 1985

N2 - Recent studies have shown that patients with a schizophrenic-like illness have a significant deficit in erythrocyte ghost membrane (EGM) phosphatidylcholine (PC); patients with the most severe deficiency showed a marked decrease in Na+-Li+ counterflow activity (Hitzemann et al. 1984a and b). The present study was undertaken to see if the decrement in PC is associated with a decrease in phospholipid methylation activity. Phospholipid methylation in human EGMs is distinctly different from that in rat EGMs (Hirata and Axelrod 1980) in that the human activity is not Mg++-dependent, and apparent methyltransferase I activity is located in the external membrane surface. The patient population consisted of 20 DSM-III schizophrenics (SCZ), 13 DSM-III schizophreniform (SF) disorder patients, and 11 DSM-III manics (M). Twelve age- and sex-matched controls were used for the comparison group. Methylation activity was significantly decreased in all three patient groups, although the M group had significantly higher activity than the SF group. Twenty-four of the SCZ and SF patients entered a Li+ trial. The Li+ responder group (n = 8) showed significantly lower activity than the nonresponder group (n = 16). Overall, we conclude that the decrement in phospholipid methylation activity partially contributes to the decrement in PC levels.

AB - Recent studies have shown that patients with a schizophrenic-like illness have a significant deficit in erythrocyte ghost membrane (EGM) phosphatidylcholine (PC); patients with the most severe deficiency showed a marked decrease in Na+-Li+ counterflow activity (Hitzemann et al. 1984a and b). The present study was undertaken to see if the decrement in PC is associated with a decrease in phospholipid methylation activity. Phospholipid methylation in human EGMs is distinctly different from that in rat EGMs (Hirata and Axelrod 1980) in that the human activity is not Mg++-dependent, and apparent methyltransferase I activity is located in the external membrane surface. The patient population consisted of 20 DSM-III schizophrenics (SCZ), 13 DSM-III schizophreniform (SF) disorder patients, and 11 DSM-III manics (M). Twelve age- and sex-matched controls were used for the comparison group. Methylation activity was significantly decreased in all three patient groups, although the M group had significantly higher activity than the SF group. Twenty-four of the SCZ and SF patients entered a Li+ trial. The Li+ responder group (n = 8) showed significantly lower activity than the nonresponder group (n = 16). Overall, we conclude that the decrement in phospholipid methylation activity partially contributes to the decrement in PC levels.

UR - http://www.scopus.com/inward/record.url?scp=0022220591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022220591&partnerID=8YFLogxK

U2 - 10.1016/0006-3223(85)90042-3

DO - 10.1016/0006-3223(85)90042-3

M3 - Article

VL - 20

SP - 397

EP - 407

JO - Biological Psychiatry

JF - Biological Psychiatry

SN - 0006-3223

IS - 4

ER -