Characteristics of a retrovirus associated with a hamster melanoma

P. Russell, D. S. Gregerson, Daniel Albert, T. W. Reid

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The continuous culture of a hamster melanoma cell line has led to the spontaneous appearance of a retrovirus (HaRV) with typical type-C characteristics. The virus difers from all other known hamster viruses in its ability to transform murine as well as rat and hamter cells with apparent one-hit kinetics. Guinea pig, human and feline cells were not transformed although reverse transcriptase activity was detected in the supernatant from infected human cells. HaRV-tansformed hamster embryo cells produced solid tumors (all non-pigmented) in 4 out of 35 animals when injected into hamsters while HaRV-transformed murine cells produced no tumors in mice. Injection of HaRV alone in hamsters, mice and rabbits did not induce tumors. HaRV possesses a 70S RNA which dissociates to 35S in DMSO and has a reverse transcriptase which utilizes the 70S virus RNA as a template. The size, morphology and density (1.15 g/ml) are similar to other known type-C viruses. Polyacrylamide gel electrophoresis indicates the presence of polypeptides analogous to those found in other type-C viruses.

Original languageEnglish (US)
Pages (from-to)317-326
Number of pages10
JournalJournal of General Virology
Volume43
Issue number2
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

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Retroviridae
Cricetinae
Melanoma
Viruses
RNA-Directed DNA Polymerase
Neoplasms
RNA Viruses
Felidae
Dimethyl Sulfoxide
Polyacrylamide Gel Electrophoresis
Guinea Pigs
Embryonic Structures
RNA
Rabbits
Cell Line
Peptides
Injections

ASJC Scopus subject areas

  • Virology

Cite this

Characteristics of a retrovirus associated with a hamster melanoma. / Russell, P.; Gregerson, D. S.; Albert, Daniel; Reid, T. W.

In: Journal of General Virology, Vol. 43, No. 2, 01.01.1979, p. 317-326.

Research output: Contribution to journalArticle

Russell, P. ; Gregerson, D. S. ; Albert, Daniel ; Reid, T. W. / Characteristics of a retrovirus associated with a hamster melanoma. In: Journal of General Virology. 1979 ; Vol. 43, No. 2. pp. 317-326.
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