Characteristics and regulation of the ovarian cycle in vervet monkeys (Chlorocebus aethiops)

Theodore A. Molskness, David Hess, Gwen M. Maginnis, Jay W. Wright, John W. Fanton, Richard Stouffer

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    This study was designed to evaluate the timecourse of ovarian and pituitary endocrine events throughout the menstrual cycle in the vervet monkey, and whether circulating luteinizing hormone (LH) or the uterus regulates the functional lifespan of the vervet corpus luteum. Daily saphenous blood samples were collected from adult females (1) during spontaneous menstrual cycles (n = 7), and (2) during cycles in which a gonadotropin-releasing hormone antagonist (acyline) was administered for 3 days at midluteal phase (n = 3), and (3) for 30 days following recovery from hysterectomy (n = 4). Estradiol (E) and progesterone (P) levels were assayed using electrochemoluminescent assays. Gonadotropin levels were measured by radioimmunoassay using reagents developed for the assay of follicle-stimulating hormone and LH in macaques. Spontaneous cycles exhibited a midcycle E rise (476 ± 49 pg/ml), engendering an LH surge, 12 ± 1 days after onset of menses, followed by a luteal phase with peak P levels of 4.7 ± 0.9 ng/ml. Histologic evaluation of the ovaries at late follicular phase or early luteal phase revealed the presence of a single, large Graafian follicle or developing corpus luteum, respectively. Acyline treatment caused a significant (P <0.05) decline in P levels (2.9 ± 0.5 vs 0.5 ± 0.3 ng/ml, 0 vs 48 h post-treatment) and premature menstruation compared with untreated controls (P <0.05). Hysterectomy had no apparent effect on the monthly pattern or levels of circulating E or P. Thus, the characteristics and regulation of the ovarian cycle in vervets appear similar to those in women and macaques, with cyclicity dependent on pituitary gonadotropin hormones and independent of a uterine luteolytic factor.

    Original languageEnglish (US)
    Pages (from-to)890-900
    Number of pages11
    JournalAmerican Journal of Primatology
    Volume69
    Issue number8
    DOIs
    StatePublished - Aug 2007

    Fingerprint

    Cercopithecus aethiops
    corpus luteum
    hormone
    luteinizing hormone
    hysterectomy
    menstruation
    menstrual cycle
    Macaca
    hormone antagonists
    Graafian follicles
    pituitary gonadotropins
    follicle-stimulating hormone
    assays
    gonadotropin-releasing hormone
    gonadotropins
    radioimmunoassays
    periodicity
    assay
    uterus
    estradiol

    Keywords

    • Estradiol
    • Gonadotropin hormones
    • Menstrual cycle
    • Progesterone
    • Uterine luteolytic factor

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Characteristics and regulation of the ovarian cycle in vervet monkeys (Chlorocebus aethiops). / Molskness, Theodore A.; Hess, David; Maginnis, Gwen M.; Wright, Jay W.; Fanton, John W.; Stouffer, Richard.

    In: American Journal of Primatology, Vol. 69, No. 8, 08.2007, p. 890-900.

    Research output: Contribution to journalArticle

    Molskness, Theodore A. ; Hess, David ; Maginnis, Gwen M. ; Wright, Jay W. ; Fanton, John W. ; Stouffer, Richard. / Characteristics and regulation of the ovarian cycle in vervet monkeys (Chlorocebus aethiops). In: American Journal of Primatology. 2007 ; Vol. 69, No. 8. pp. 890-900.
    @article{96c635c7717f44d1880754602e767fa0,
    title = "Characteristics and regulation of the ovarian cycle in vervet monkeys (Chlorocebus aethiops)",
    abstract = "This study was designed to evaluate the timecourse of ovarian and pituitary endocrine events throughout the menstrual cycle in the vervet monkey, and whether circulating luteinizing hormone (LH) or the uterus regulates the functional lifespan of the vervet corpus luteum. Daily saphenous blood samples were collected from adult females (1) during spontaneous menstrual cycles (n = 7), and (2) during cycles in which a gonadotropin-releasing hormone antagonist (acyline) was administered for 3 days at midluteal phase (n = 3), and (3) for 30 days following recovery from hysterectomy (n = 4). Estradiol (E) and progesterone (P) levels were assayed using electrochemoluminescent assays. Gonadotropin levels were measured by radioimmunoassay using reagents developed for the assay of follicle-stimulating hormone and LH in macaques. Spontaneous cycles exhibited a midcycle E rise (476 ± 49 pg/ml), engendering an LH surge, 12 ± 1 days after onset of menses, followed by a luteal phase with peak P levels of 4.7 ± 0.9 ng/ml. Histologic evaluation of the ovaries at late follicular phase or early luteal phase revealed the presence of a single, large Graafian follicle or developing corpus luteum, respectively. Acyline treatment caused a significant (P <0.05) decline in P levels (2.9 ± 0.5 vs 0.5 ± 0.3 ng/ml, 0 vs 48 h post-treatment) and premature menstruation compared with untreated controls (P <0.05). Hysterectomy had no apparent effect on the monthly pattern or levels of circulating E or P. Thus, the characteristics and regulation of the ovarian cycle in vervets appear similar to those in women and macaques, with cyclicity dependent on pituitary gonadotropin hormones and independent of a uterine luteolytic factor.",
    keywords = "Estradiol, Gonadotropin hormones, Menstrual cycle, Progesterone, Uterine luteolytic factor",
    author = "Molskness, {Theodore A.} and David Hess and Maginnis, {Gwen M.} and Wright, {Jay W.} and Fanton, {John W.} and Richard Stouffer",
    year = "2007",
    month = "8",
    doi = "10.1002/ajp.20395",
    language = "English (US)",
    volume = "69",
    pages = "890--900",
    journal = "American Journal of Primatology",
    issn = "0275-2565",
    publisher = "John Wiley and Sons Inc.",
    number = "8",

    }

    TY - JOUR

    T1 - Characteristics and regulation of the ovarian cycle in vervet monkeys (Chlorocebus aethiops)

    AU - Molskness, Theodore A.

    AU - Hess, David

    AU - Maginnis, Gwen M.

    AU - Wright, Jay W.

    AU - Fanton, John W.

    AU - Stouffer, Richard

    PY - 2007/8

    Y1 - 2007/8

    N2 - This study was designed to evaluate the timecourse of ovarian and pituitary endocrine events throughout the menstrual cycle in the vervet monkey, and whether circulating luteinizing hormone (LH) or the uterus regulates the functional lifespan of the vervet corpus luteum. Daily saphenous blood samples were collected from adult females (1) during spontaneous menstrual cycles (n = 7), and (2) during cycles in which a gonadotropin-releasing hormone antagonist (acyline) was administered for 3 days at midluteal phase (n = 3), and (3) for 30 days following recovery from hysterectomy (n = 4). Estradiol (E) and progesterone (P) levels were assayed using electrochemoluminescent assays. Gonadotropin levels were measured by radioimmunoassay using reagents developed for the assay of follicle-stimulating hormone and LH in macaques. Spontaneous cycles exhibited a midcycle E rise (476 ± 49 pg/ml), engendering an LH surge, 12 ± 1 days after onset of menses, followed by a luteal phase with peak P levels of 4.7 ± 0.9 ng/ml. Histologic evaluation of the ovaries at late follicular phase or early luteal phase revealed the presence of a single, large Graafian follicle or developing corpus luteum, respectively. Acyline treatment caused a significant (P <0.05) decline in P levels (2.9 ± 0.5 vs 0.5 ± 0.3 ng/ml, 0 vs 48 h post-treatment) and premature menstruation compared with untreated controls (P <0.05). Hysterectomy had no apparent effect on the monthly pattern or levels of circulating E or P. Thus, the characteristics and regulation of the ovarian cycle in vervets appear similar to those in women and macaques, with cyclicity dependent on pituitary gonadotropin hormones and independent of a uterine luteolytic factor.

    AB - This study was designed to evaluate the timecourse of ovarian and pituitary endocrine events throughout the menstrual cycle in the vervet monkey, and whether circulating luteinizing hormone (LH) or the uterus regulates the functional lifespan of the vervet corpus luteum. Daily saphenous blood samples were collected from adult females (1) during spontaneous menstrual cycles (n = 7), and (2) during cycles in which a gonadotropin-releasing hormone antagonist (acyline) was administered for 3 days at midluteal phase (n = 3), and (3) for 30 days following recovery from hysterectomy (n = 4). Estradiol (E) and progesterone (P) levels were assayed using electrochemoluminescent assays. Gonadotropin levels were measured by radioimmunoassay using reagents developed for the assay of follicle-stimulating hormone and LH in macaques. Spontaneous cycles exhibited a midcycle E rise (476 ± 49 pg/ml), engendering an LH surge, 12 ± 1 days after onset of menses, followed by a luteal phase with peak P levels of 4.7 ± 0.9 ng/ml. Histologic evaluation of the ovaries at late follicular phase or early luteal phase revealed the presence of a single, large Graafian follicle or developing corpus luteum, respectively. Acyline treatment caused a significant (P <0.05) decline in P levels (2.9 ± 0.5 vs 0.5 ± 0.3 ng/ml, 0 vs 48 h post-treatment) and premature menstruation compared with untreated controls (P <0.05). Hysterectomy had no apparent effect on the monthly pattern or levels of circulating E or P. Thus, the characteristics and regulation of the ovarian cycle in vervets appear similar to those in women and macaques, with cyclicity dependent on pituitary gonadotropin hormones and independent of a uterine luteolytic factor.

    KW - Estradiol

    KW - Gonadotropin hormones

    KW - Menstrual cycle

    KW - Progesterone

    KW - Uterine luteolytic factor

    UR - http://www.scopus.com/inward/record.url?scp=34548044503&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=34548044503&partnerID=8YFLogxK

    U2 - 10.1002/ajp.20395

    DO - 10.1002/ajp.20395

    M3 - Article

    VL - 69

    SP - 890

    EP - 900

    JO - American Journal of Primatology

    JF - American Journal of Primatology

    SN - 0275-2565

    IS - 8

    ER -