Characterisation of seven human ovarian tumour cell lines

A. P. Wilson; M. Dent; T. Pejovic; L. Hubbold; H. Radford

doi:10.1038/bjc.1996.428

Characterisation of seven human ovarian tumour cell lines

A. P. Wilson, M. Dent, T. Pejovic, L. Hubbold, H. Radford

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Characteristics of a panel of seven human ovarian tumour cell lines are presented. Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines. The lines showed wide variations in ploidy, doubling times and clonogenicity in soft agar. Both vimentin and keratin were equally expressed in five lines, one line showed strong preferential expression of keratin and one line showed preferential expression of vimentin. Karyotypic changes associated with ovarian cancer were identified in all the lines. Four of the seven cell lines showed loss of chromosome material distal to 11p13-15. These cell lines offer considerable potential for research into the biology and genetics of ovarian cancer.

Original language	English (US)
Pages (from-to)	722-727
Number of pages	6
Journal	British Journal of Cancer
Volume	74
Issue number	5
DOIs	https://doi.org/10.1038/bjc.1996.428
State	Published - 1996
Externally published	Yes

Keywords

Characterisation
Heterogeneity
Karyotype
Ovarian tumour line

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1038/bjc.1996.428

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title = "Characterisation of seven human ovarian tumour cell lines",

abstract = "Characteristics of a panel of seven human ovarian tumour cell lines are presented. Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines. The lines showed wide variations in ploidy, doubling times and clonogenicity in soft agar. Both vimentin and keratin were equally expressed in five lines, one line showed strong preferential expression of keratin and one line showed preferential expression of vimentin. Karyotypic changes associated with ovarian cancer were identified in all the lines. Four of the seven cell lines showed loss of chromosome material distal to 11p13-15. These cell lines offer considerable potential for research into the biology and genetics of ovarian cancer.",

keywords = "Characterisation, Heterogeneity, Karyotype, Ovarian tumour line",

author = "Wilson, {A. P.} and M. Dent and T. Pejovic and L. Hubbold and H. Radford",

note = "Funding Information: The co-operation of medical and theatre staff in providing clinical material is appreciated. Electron microscopy was kindly done by Dr P Fryer, Clinical Research Centre, Harrow. The work was funded by the people of Derby through donations to the Cancer Research (Immunology) Trust Fund and by the Southern Derbyshire Health Authority. The support of Dr PR Golding (Medical Director and Founder of the Laboratory) is gratefully acknowledged.",

year = "1996",

doi = "10.1038/bjc.1996.428",

language = "English (US)",

volume = "74",

pages = "722--727",

journal = "British Journal of Cancer",

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number = "5",

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T1 - Characterisation of seven human ovarian tumour cell lines

AU - Wilson, A. P.

AU - Dent, M.

AU - Pejovic, T.

AU - Hubbold, L.

AU - Radford, H.

N1 - Funding Information: The co-operation of medical and theatre staff in providing clinical material is appreciated. Electron microscopy was kindly done by Dr P Fryer, Clinical Research Centre, Harrow. The work was funded by the people of Derby through donations to the Cancer Research (Immunology) Trust Fund and by the Southern Derbyshire Health Authority. The support of Dr PR Golding (Medical Director and Founder of the Laboratory) is gratefully acknowledged.

PY - 1996

Y1 - 1996

N2 - Characteristics of a panel of seven human ovarian tumour cell lines are presented. Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines. The lines showed wide variations in ploidy, doubling times and clonogenicity in soft agar. Both vimentin and keratin were equally expressed in five lines, one line showed strong preferential expression of keratin and one line showed preferential expression of vimentin. Karyotypic changes associated with ovarian cancer were identified in all the lines. Four of the seven cell lines showed loss of chromosome material distal to 11p13-15. These cell lines offer considerable potential for research into the biology and genetics of ovarian cancer.

AB - Characteristics of a panel of seven human ovarian tumour cell lines are presented. Positive staining with HMFG2 and ultrastructural identification of desmosomes confirmed the epithelial nature of the cell lines. The lines showed wide variations in ploidy, doubling times and clonogenicity in soft agar. Both vimentin and keratin were equally expressed in five lines, one line showed strong preferential expression of keratin and one line showed preferential expression of vimentin. Karyotypic changes associated with ovarian cancer were identified in all the lines. Four of the seven cell lines showed loss of chromosome material distal to 11p13-15. These cell lines offer considerable potential for research into the biology and genetics of ovarian cancer.

KW - Characterisation

KW - Heterogeneity

KW - Karyotype

KW - Ovarian tumour line

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Characterisation of seven human ovarian tumour cell lines

Abstract

Keywords

ASJC Scopus subject areas

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