Changes in the concentration of activation factor for phosphofructokinase in hepatocytes in response to glucose and glucagon

Carolyn Sue Richards, Kosaku Uyeda

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Recently we discovered "activation factor" which prevents ATP inhibition of liver phosphofructokinase (Proc. Natl. Acad. Sci., in press). The effect of glucagon and glucose administration on the levels of the factor was investigated using isolated hepatocytes. The concentration of the factor in hepatocytes increased at least 15-fold within 8 min after administration of 20 mM glucose. Upon glucagon administration (10-7M), the level of activation factor decreased rapidly and within 2 min the amount decreased to 1/13 of the original concentration. It is proposed that glucagon acts to inhibit phosphofructokinase in the liver by (1) decreasing the level of activation factor, and (2) phosphorylation of the enzyme.

Original languageEnglish (US)
Pages (from-to)1535-1540
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume97
Issue number4
DOIs
StatePublished - Dec 31 1980

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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