Changes in proteasome expression and activity during differentiation of neuronal precursor NTera 2 clone D1 cells

Cezary Wójcik, Sherwin Wilk

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The effect of differentiation of the human neuronal progenitor cell line NTera 2 clone D1 (NT2/D1) by retinoic acid on components of the proteasome system was studied. The chymotrypsin-like and peptidylglutamyl peptide bond hydrolyzing activities of the proteasome increased five weeks after retinoic acid, and following treatment with mitotic inhibitors returned to levels detected in non-differentiated cells. A selective induction of the MHC class II region encoded LMP7 and LMP2 proteasome subunits occurred during differentiation, whereas there were no changes in the expression of the constitutive LMP2 counterpart (δ-subunit) or the constitutive C2 subunit. Immunofluorescence revealed marked LMP7 accumulation in fully differentiated cells, with no changes in the labeling pattern of the constitutive proteasome antigens. The expression of the Y-subunit of the PA28 proteasome activator was down-regulated in fully differentiated neurons, but was not correlated with changes in enzymatic activity. Changes in proteasome activity and composition may contribute to the processes leading to differentiation of human neurons in vitro and to the properties of fully differentiated neurons.

Original languageEnglish (US)
Pages (from-to)131-136
Number of pages6
JournalNeurochemistry International
Volume34
Issue number2
DOIs
StatePublished - 1999

Keywords

  • LMP
  • Neuronal differentiation
  • PA28
  • Proteasomes
  • Retinoic acid

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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