Changes in L-ornithine decarboxylase activity during the cell cycle

Olle Heby, Joe Gray, Patricia A. Lindl, Laurence J. Marton, Charles B. Wilson

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The activity of L-ornithine decarboxylase (L-ornithine carboxy-lyase; EC 4.1.1.17), the enzyme that catalyzes the initial and rate-limiting step in polyamine biosynthesis, has been studied in Chinese hamster ovary fibroblasts synchronized by selective detachment of mitotic cells. At various times after plating the distribution of cells among the G1, S and G2+M phases of the cell cycle was calculated from DNA distributions obtained by high-speed flow cytometric analysis. At these same times determination of the cellular L-ornithine decarboxylase activity showed that polyamine (putrescine) synthesis was initiated in mid-G1, that the rate of synthesis was maximal prior to DNA synthesis, and that it decreased during the S phase. A second increase in enzyme activity occurred before mitosis.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume71
Issue number1
DOIs
StatePublished - Jul 12 1976
Externally publishedYes

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Ornithine Decarboxylase
Cell Cycle
Cells
Polyamines
Putrescine
DNA
Biosynthesis
Enzyme activity
Fibroblasts
Plating
G2 Phase
Enzymes
Cricetulus
S Phase
Mitosis
Cell Division
Ovary

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Changes in L-ornithine decarboxylase activity during the cell cycle. / Heby, Olle; Gray, Joe; Lindl, Patricia A.; Marton, Laurence J.; Wilson, Charles B.

In: Biochemical and Biophysical Research Communications, Vol. 71, No. 1, 12.07.1976, p. 99-105.

Research output: Contribution to journalArticle

Heby, Olle ; Gray, Joe ; Lindl, Patricia A. ; Marton, Laurence J. ; Wilson, Charles B. / Changes in L-ornithine decarboxylase activity during the cell cycle. In: Biochemical and Biophysical Research Communications. 1976 ; Vol. 71, No. 1. pp. 99-105.
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