Changes in expression of vascular endothelial growth factor and angiopoietin-1 and -2 in the macaque corpus luteum during the menstrual cycle

T. M. Hazzard, L. K. Christenson, Richard Stouffer

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    To determine the temporal expression of vascular growth factors during the lifespan of the primate corpus luteum, experiments were designed to detect mRNA for vascular endothelial growth factor (VEGF), angiopoietin (Ang)-1 and Ang-2 and to localize protein expression for VEGF in macaque luteal tissue during the menstrual cycle. Corpora lutea (n = 3-5/stage) were collected during the early (3-5 days post-luteinizing hormone surge), mid- (6-8 days), mid-late (10-12 days), and late (14-16 days) luteal phase and at menstruation (17-18 days). Reverse transcription-polymerase chain reaction products equated to cDNA for VEGF, Ang-1 and Ang-2 in all corpora lutea. VEGF mRNA levels increased (P <0.05) from early to mid-luteal phase and declined in the late luteal phase and at menstruation. Immunostaining for VEGF was detected in the cytoplasm of steroidogenic luteal cells, with the most intense staining in the early luteal phase. Ang-1 and Ang-2 mRNA expression was low in the early to mid-luteal phase but increased (P <0.05) at late luteal phase before declining at menstruation. These data suggest transcriptional control of VEGF, Ang-1 and Ang-2, as well as post-transcriptional control of VEGF, in macaque corpus luteum. Dynamic expression of angiogenic/angiostatic factors appears critical for development, maintenance and regression of the luteal microvasculature during the menstrual cycle.

    Original languageEnglish (US)
    Pages (from-to)993-998
    Number of pages6
    JournalMolecular Human Reproduction
    Volume6
    Issue number11
    StatePublished - 2000

    Fingerprint

    Angiopoietin-2
    Angiopoietin-1
    Corpus Luteum
    Macaca
    Menstrual Cycle
    Luteal Phase
    Vascular Endothelial Growth Factor A
    Menstruation
    Messenger RNA
    Corpus Luteum Maintenance
    Luteolysis
    Luteal Cells
    Angiogenesis Inducing Agents
    Luteinizing Hormone
    Microvessels
    Primates
    Reverse Transcription
    Intercellular Signaling Peptides and Proteins
    Cytoplasm
    Complementary DNA

    Keywords

    • Angiogenesis
    • Angiopoietin-1
    • Angiopoietin-2
    • Corpus luteum
    • Vascular endothelial growth factor

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Genetics
    • Developmental Biology
    • Embryology
    • Cell Biology

    Cite this

    Changes in expression of vascular endothelial growth factor and angiopoietin-1 and -2 in the macaque corpus luteum during the menstrual cycle. / Hazzard, T. M.; Christenson, L. K.; Stouffer, Richard.

    In: Molecular Human Reproduction, Vol. 6, No. 11, 2000, p. 993-998.

    Research output: Contribution to journalArticle

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