Changes in activity of cytosolic phospholipase A2 in human amnion at parturition

D. G. Skannal, D. E. Brockman, A. L.W. Eis, S. Xue, T. A. Siddiqi, L. Myatt

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


OBJECTIVE: The purpose of this study was to determine whether increased cytosolic phospholipase A2 activity mediated arachidonic acid mobilization for prostaglandin synthesis in amnion at parturition. STUDY DESIGN: Amnion was collected immediately after delivery from four groups of patients: preterm (<37 weeks) with no labor or labor and term (>37 weeks) with no labor or labor and stored at -70°C. Tissues were homogenized and centrifuged for 1 hour at 100,000g, and cytosol was assayed for cytosolic phospholipase A2 activity with use of carbon 14-labeled 1-stearoyl-2 arachidonyl phosphatidylcholine plus 10 μmol/L unlabeled substrate and 5 mmol/L calcium in 10 mmol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid, pH 7.4. Incubations were performed in duplicate ± 10 μmol/L arachidonyl trifluoromethyl ketone, a specific inhibitor of cytosolic phospholipase A2 activity, at 30°C for 45 minutes. RESULTS: Total cytosolic phospholipase A2 activity (in picomoles of arachidonic acid per minute per milligram of protein) calculated as the difference between the activity in the presence and absence of arachidonyl trifluoromethyl ketone was (mean ± SE) as follows: preterm no labor (n = 7) 8.94 ± 3.08, preterm with labor (n = 6) 6.79 ± 2.31, term no labor (n = 7) 14.85 ± 1.66, and term with labor (n = 5) 5.51 ± 1.52. Enzyme activity increased with gestational age and was highest in the term no labor group. A significant decrease in cytosolic phospholipase A2 activity occurred with labor (p < 0.05). The greatest decrease in activity was in the term group (p < 0.05). CONCLUSION: Total cellular cytosolic phospholipase A2 activity in amnion is highest in anticipation of labor but during labor total activity is depleted, resulting in the low activity measured after delivery of the placenta. The substrate specificity and changes in amnion total cytosolic phospholipase A2 activity with labor strongly suggests a role in mediation of arachidonic acid mobilization and prostaglandin synthesis at labor.

Original languageEnglish (US)
Pages (from-to)179-184
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Issue number1
StatePublished - 1997
Externally publishedYes


  • Amnion
  • Cytosolic phospholipase A
  • Parturition
  • Prostaglandin

ASJC Scopus subject areas

  • Obstetrics and Gynecology


Dive into the research topics of 'Changes in activity of cytosolic phospholipase A2 in human amnion at parturition'. Together they form a unique fingerprint.

Cite this